Discontinued Product
3174 has been discontinued.
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Product Description
HEPES Sodium salt is a multi purpose buffer used in biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
Product Specifications for HEPES Sodium salt
Molecular Weight
260.29
Formula
C8H17N2NaO4S
Storage
Store at RT
Purity
≥99.5%
Chemical Name
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt
CAS Number
75277-39-3
PubChem ID
2724248
InChI Key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
SMILES
[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Calculators
Background References
References are publications that support the biological activity of the product.
- Medzon and Gedies Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol.
- Good Hydrogen ion buffers for biological research. Biochemistry 1966 PMID: 5942950
Product Documents for HEPES Sodium salt
Product Specific Notices for HEPES Sodium salt
For research use only
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Species: HumanAssay Type: In VitroCell Line/Tissue: HEK293Verified Customer | Posted 02/04/2019HEPES Sodium salt used to make buffer for HEK 293 cell culture. DMEM (Dulbecco’s Minimal Essential Media) with 10 % foetal calf serum (FCS) used as media.
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