HEPES Sodium salt
Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt
Biological ActivityHEPES Sodium salt is a multi purpose buffer used in biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Hydrogen ion buffers for biological research.
Good et al.
Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
Medzon and Gedies
Canadian J.Microbiol., 1971;17:651
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HEPES Sodium salt used to make buffer for HEK 293 cell culture. DMEM (Dulbecco’s Minimal Essential Media) with 10 % foetal calf serum (FCS) used as media.