HEPES Sodium salt

Tocris Bioscience | Catalog # 3174

Multipurpose buffer
Tocris Bioscience
Discontinued Product
3174 has been discontinued. View all Buffers, Chelators and Reagents products.

Key Product Details

Description

Multipurpose buffer

Product Description

HEPES Sodium salt is a multi purpose buffer used in biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Product Specifications for HEPES Sodium salt

Molecular Weight

260.29

Formula

C8H17N2NaO4S

Storage

Store at RT

Purity

≥99.5%

Chemical Name

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt

CAS Number

75277-39-3

PubChem ID

2724248

InChI Key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

SMILES

[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

  • Medzon and Gedies Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol.
  • Good Hydrogen ion buffers for biological research. Biochemistry 1966 PMID: 5942950

Product Documents for HEPES Sodium salt

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HEPES Sodium salt

For research use only

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  • HEPES Sodium salt used to make buffer for HEK 293 cell culture: Gives good buffer pH
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 02/04/2019
    HEPES Sodium salt used to make buffer for HEK 293 cell culture. DMEM (Dulbecco’s Minimal Essential Media) with 10 % foetal calf serum (FCS) used as media.
    HEPES Sodium salt 3174

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