HEPES

Catalog # Availability Size / Price Qty
3173/250G
Cat.No. 3173 - HEPES | C8H18N2O4S | CAS No. 7365-45-9
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Description: Multipurpose buffer

Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

Purity: ≥99.5%

Product Details
Citations (1)
Reviews (4)

Biological Activity

Multi purpose buffer used in biological research.

Technical Data

M.Wt:
238.3
Formula:
C8H18N2O4S
Solubility:
Soluble to 1000 mM in water
Purity:
≥99.5%
Storage:
Store at RT
CAS No:
7365-45-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.

Background References

  1. Hydrogen ion buffers for biological research.
    Good et al.
    Biochemistry, 1966;5:467
  2. Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
    Medzon and Gedies
    Canadian J.Microbiol., 1971;17:651

Product Datasheets

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Citation for HEPES

The citations listed below are publications that use Tocris products. Selected citations for HEPES include:

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FAQs

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Reviews for HEPES

Average Rating: 4.8 (Based on 4 Reviews)

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Primary cell culture
By Anonymous on 09/04/2018
Species: Mouse

Used at 20 mmol/L in DMEM buffer for primary cell culture


Good buffer
By Anonymous on 08/05/2018
Species: Mouse

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.


Media ingredient
By Anonymous on 05/01/2018
Species: Mouse

Additive in cell culture media


HEPES to buffer pH
By Anonymous on 01/31/2018
Species: Mouse

HEPES used in solutions, good pH buffer.


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