HEPES

Catalog # Availability Size / Price Qty
3173/250G
HEPES | CAS No. 7365-45-9 | Buffers, Chelators and Reagents
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Description: Multipurpose HEPES buffer; used in cell culture

Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

Purity: ≥99.5%

Product Details
Citations (1)
Reviews (2)

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

For more information about how HEPES may be used, see our protocol:

3D Culture of Lung Alveolar Cells

Technical Data

M.Wt:
238.3
Formula:
C8H18N2O4S
Solubility:
Soluble to 1000 mM in water
Purity:
≥99.5%
Storage:
Store at RT
CAS No:
7365-45-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Hydrogen ion buffers for biological research.
    Good et al.
    Biochemistry, 1966;5:467
  2. Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
    Medzon and Gedies
    Canadian J.Microbiol., 1971;17:651

Product Datasheets

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Citation for HEPES

The citations listed below are publications that use Tocris products. Selected citations for HEPES include:

1 Citation: Showing 1 - 1

FAQs

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Reviews for HEPES

Average Rating: 5 (Based on 2 Reviews)

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Primary cell culture
By Oonagh Paerewijck on 09/04/2018

Used at 20 mmol/L in DMEM buffer for primary cell culture


Media ingredient
By Danlee Enzler on 05/01/2018

Additive in cell culture media


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