Catalog # Availability Size / Price Qty
HEPES | CAS No. 7365-45-9 | Buffers, Chelators and Reagents
1 Image
Description: Multipurpose HEPES buffer; used in cell culture

Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

Purity: ≥99.5%

Product Details
Citations (1)
Reviews (5)

Biological Activity

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

Technical Data

Soluble to 1000 mM in water
Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Hydrogen ion buffers for biological research.
    Good et al.
    Biochemistry, 1966;5:467
  2. Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
    Medzon and Gedies
    Canadian J.Microbiol., 1971;17:651

Product Datasheets

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Citation for HEPES

The citations listed below are publications that use Tocris products. Selected citations for HEPES include:

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Reviews for HEPES

Average Rating: 4.6 (Based on 5 Reviews)

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HEPES (10 mM) maintains pH in the experimental buffer for Ca2+ imaging.
By Anonymous on 07/02/2019
Application: Species: Human

HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.

Primary cell culture
By Anonymous on 09/04/2018
Application: Species: Mouse

Used at 20 mmol/L in DMEM buffer for primary cell culture

Good buffer
By Anonymous on 08/05/2018
Application: Species: Mouse

Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.

Media ingredient
By Anonymous on 05/01/2018
Application: Species: Mouse

Additive in cell culture media

HEPES to buffer pH
By Anonymous on 01/31/2018
Application: Species: Mouse

HEPES used in solutions, good pH buffer.

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