Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Biological ActivityHEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
For more information about how HEPES may be used, see our protocol:3D Culture of Lung Alveolar Cells
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Hydrogen ion buffers for biological research.
Good et al.
Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
Medzon and Gedies
Canadian J.Microbiol., 1971;17:651
Citation for HEPES
The citations listed below are publications that use Tocris products. Selected citations for HEPES include:
1 Citation: Showing 1 - 1
Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists.
Authors: Lerner Et al.
J Neurosci 2010;30:2160
No product specific FAQs exist for this product, however you mayView all Small Molecule FAQs
Reviews for HEPES
Average Rating: 5 (Based on 2 Reviews)
Have you used HEPES?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Used at 20 mmol/L in DMEM buffer for primary cell culture
Additive in cell culture media