Chemical Name: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Biological ActivityMulti purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Hydrogen ion buffers for biological research.
Good et al.
Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells.
Medzon and Gedies
Canadian J.Microbiol., 1971;17:651
Citation for HEPES
The citations listed below are publications that use Tocris products. Selected citations for HEPES include:
1 Citation: Showing 1 - 1
Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists.
Authors: Lerner Et al.
J Neurosci 2010;30:2160
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Reviews for HEPES
Average Rating: 4.6 (Based on 5 Reviews)
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HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.
Used at 20 mmol/L in DMEM buffer for primary cell culture
Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.
Additive in cell culture media
HEPES used in solutions, good pH buffer.