Histamine ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62860

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL
View all Histamine ELISA Kits »

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

This kit recognizes HIS in samples. No significant cross-reactivity or interference between HIS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.66%

Inter-Assay Precision (Precision between assays) CV% 4.58%

Recovery for Histamine ELISA Kit (Colorimetric)

Recovery

87-105%

Linearity

Histamine ELISA Kit (Colorimetric)

Scientific Data Images for Histamine ELISA Kit (Colorimetric)

Histamine ELISA Kit (Colorimetric)

ELISA: Histamine ELISA Kit (Colorimetric) [NBP2-62860] -

ELISA: Histamine ELISA Kit (Colorimetric) [NBP2-62860] - Standard Curve Reference

Kit Contents for Histamine ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Histamine

Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.

Alternate Names

2 (4 imidazolyl)ethylamine, 4 (2 aminoethyl) 1 3 diazole

Additional Histamine Products

Product Documents for Histamine ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Histamine ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Histamine ELISA Kit (Colorimetric)

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