Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Histone H2AX Antibody (CL5157) - BSA Free
Novus Biologicals | Catalog # NBP3-21157
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL5157
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from P16104, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Histone H2AX Antibody (CL5157) - BSA Free
![Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -](https://resources.rndsystems.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315493273.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -")
Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21157] -
Staining of MCF7 cells using the Anti-H2AFX monoclonal antibody, showing specific staining in nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.rndsystems.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-4520231548527.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -
Staining of human endometrium shows strong nuclear immunoreactivity in glandular and stromal cells.![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.rndsystems.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315472814.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -
Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.rndsystems.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315534342.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -
Staining of human kidney shows strong nuclear positivity in renal tubules cells.![Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -](https://resources.rndsystems.com/images/products/nbp3-21157_mouse-histone-h2ax-mab-cl5157-45202315531392.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -")
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-21157] -
Staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.Applications for Histone H2AX Antibody (CL5157) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:20000 - 1:50000
Immunohistochemistry-Paraffin
1:20000 - 1:50000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2AX
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (CL5157) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H2AX Antibody (CL5157) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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