Home / Amine-Reactive Janelia Fluor Dyes / HM Janelia Fluor® 526, SE

HM Janelia Fluor® 526, SE

Catalog #: 7312 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7312/1
HM Janelia Fluor® 526, SE | CAS No. 2376841-30-2 | Amine-Reactive Janelia Fluor Dyes
1 Image
Description: Fluorogenic spontaneously blinking yellow-emitting dye; for SMLSM; λabsλem 526/550 nm
Alternative Names: HM-JF526 NHS

Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate

Purity: ≥90%

Product Details
Reviews
Biological Activity Technical Data Additional Information Background Product Datasheets Calculators

Biological Activity

HM Janelia Fluor® 526, SE is a fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Optical Data for HM Janelia Fluor® 526, SE

HM Janelia Fluor<sup>®</sup> 526, SE Dye Spectra
Emission Color Yellow
Brightness
λabs 526 nm
λem 550 nm
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application dSTORM, SMLSM, STED, SIM, confocal microscopy

Technical Data

M.Wt:
645.51
Formula:
C31H21F6N3O6
Solubility:
Soluble to 20 mM in DMSO
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
2376841-30-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Molarity Calculator

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for HM Janelia Fluor® 526, SE

There are currently no reviews for this product. Be the first to review HM Janelia Fluor® 526, SE and earn rewards!

Have you used HM Janelia Fluor® 526, SE?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.