HM Janelia Fluor® 526, SE

Catalog # Availability Size / Price Qty
HM Janelia Fluor® 526, SE | CAS No. 2376841-30-2 | Amine-Reactive Janelia Fluor Dyes
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Description: Fluorogenic spontaneously blinking yellow-emitting dye; for SMLSM; λabsλem 526/550 nm
Alternative Names: HM-JF526 NHS

Chemical Name: 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate

Purity: ≥90%

Product Details

Biological Activity

HM Janelia Fluor® 526, SE is a fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®.

To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Optical Data for HM Janelia Fluor® 526, SE

HM Janelia Fluor<sup>®</sup> 526, SE Dye Spectra
Emission Color Yellow
λabs 526 nm
λem 550 nm
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Application dSTORM, SMLSM, STED, SIM, confocal microscopy

Technical Data

Soluble to 20 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).


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