Recombinant Human HMGB1/HMG-1 His Protein
Novus Biologicals | Catalog # NBC1-18488
![SDS-PAGE: Recombinant Human HMGB1/HMG-1 His Protein [NBC1-18488]](https://resources.rndsystems.com/images/products/Recombinant-Human-HMGB1-HMG-1-Protein-SDS-Page-NBC1-18488-img0002.jpg "SDS-PAGE: Recombinant Human HMGB1/HMG-1 His Protein [NBC1-18488]")
Key Product Details
Source
Tag
Applications
Product Specifications
Description
Source: Baculovirus
Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH
Purity
Endotoxin Level
Predicted Molecular Mass
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Scientific Data Images for Recombinant Human HMGB1/HMG-1 His Protein
SDS-PAGE: Recombinant Human HMGB1/HMG-1 His Protein [NBC1-18488]
SDS-Page: Recombinant Human HMGB1/HMG-1 Protein [NBC1-18488] - HMGB1, 25kDa (223aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE.Formulation, Preparation, and Storage
NBC1-18488
| Formulation | 20 mM Tris-HCl buffer (pH 8.0), 1 mM EDTA, 0.5 MM DTT, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: HMGB1/HMG-1
Long Name
Alternate Names
Gene Symbol
Additional HMGB1/HMG-1 Products
Product Documents for Recombinant Human HMGB1/HMG-1 His Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Recombinant Human HMGB1/HMG-1 His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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FAQs for Recombinant Human HMGB1/HMG-1 His Protein
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Q: Are both of these proteins any carrier protein free? HMG1L10 Protein (Catalog # P3789) and HMGB1 Protein (catalog # NBC1-18488)
A: These proteins are carrier free. Catalog # P3789 is supplied in 50 mM HEPES, 100mM NaCl, pH 7.5 (30% glycerol, 30 mM Glutathione, 0.5% TritonX-100, 1 mM dithiothreitol). Catalog #NBC1-18488 is supplied in liquid in 20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol.
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Q: I am looking for the recombinant human HMGB-1 which we would like to use for cultured human intervertebral disc cells in vitro. 1)Is your HMGB-1 protein NBC1-18488 already sterilized? 2)Are there some articles in which NBC1-18488 is used for human cultured cells in vitro?
A: NBC1-18488 is not sterilized, and may not be suitable for use in live cells. We have only approved this protein to work for ELISA and western blot, and have not assessed its biological activity. As such, we do not have any references that cite this product in cell culture.
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Q: Are both of these proteins any carrier protein free? HMG1L10 Protein (Catalog # P3789) and HMGB1 Protein (catalog # NBC1-18488)
A: These proteins are carrier free. Catalog # P3789 is supplied in 50 mM HEPES, 100mM NaCl, pH 7.5 (30% glycerol, 30 mM Glutathione, 0.5% TritonX-100, 1 mM dithiothreitol). Catalog #NBC1-18488 is supplied in liquid in 20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol.
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Q: I am looking for the recombinant human HMGB-1 which we would like to use for cultured human intervertebral disc cells in vitro. 1)Is your HMGB-1 protein NBC1-18488 already sterilized? 2)Are there some articles in which NBC1-18488 is used for human cultured cells in vitro?
A: NBC1-18488 is not sterilized, and may not be suitable for use in live cells. We have only approved this protein to work for ELISA and western blot, and have not assessed its biological activity. As such, we do not have any references that cite this product in cell culture.