HRAS Antibody (4Q7M5)

Novus Biologicals | Catalog # NBP3-15818

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Q7M5 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 90-189 of human HRAS (P01112). FEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HRAS Antibody (4Q7M5)

Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818]

Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818]

Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818] - Western blot analysis of extracts of various cell lines, using HRAS antibody (NBP3-15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818]

Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818]

Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818] - Immunohistochemistry of paraffin-embedded human breast cancer using HRAS antibody (NBP3-15818) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for HRAS Antibody (4Q7M5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HRAS

Alternate Names

C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1

Gene Symbol

HRAS

Additional HRAS Products

Product Documents for HRAS Antibody (4Q7M5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HRAS Antibody (4Q7M5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HRAS Antibody (4Q7M5)

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Protocols

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