Recombinant Human HSP27 Protein

Novus Biologicals | Catalog # NBC1-18364

Novus Biologicals
100% Guarantee

Key Product Details

Source

E. coli

Applications

SDS-PAGE
View all HSP27 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to amino acids 1 to 205 of Human HSP27

Source: E.coli

Amino Acid Sequence: MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBC1-18364 in the following applications:

Scientific Data Images for Recombinant Human HSP27 Protein

SDS-PAGE: Recombinant Human HSP27 Protein [NBC1-18364]

SDS-PAGE: Recombinant Human HSP27 Protein [NBC1-18364]

SDS-Page: Recombinant Human HSP27 Protein [NBC1-18364] - HSP27, 22.7kDa (205aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation, and Storage

NBC1-18364
Formulation 20 mM HEPES buffer (pH 7.5), 100 mM KCl, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP27

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for Recombinant Human HSP27 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human HSP27 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human HSP27 Protein

Customer Reviews for Recombinant Human HSP27 Protein (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human HSP27 Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Recombinant Human HSP27 Protein
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 10/11/2023

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes