PARP/Apoptosis Colorimetric Assay Kit

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.

A: RIPA buffer is not recommended for use in the PARP/Apoptosis Colorimetric Assay. The recommended lysis buffer for this assay is the Cell Extraction Buffer described in the insert.

A: Protease inhibitor PMSF has been validated for use in this kit. Other protease inhibitors may be suitable and would require end user evaluation.

A: Yes, the kit is designed for multiple species including human samples.

A: This kit recognizes PAR polymers 2 to 50 units long. 

A: R&D Systems strongly recommends including a standard curve in every assay. The PARP Standard Curve verifies the assay is working properly and allows the user to express the level of PARP activity in their extract as PARP units per amount of protein or PARP units per cell number.

A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

A: We recommend using less than 5 uM of etoposide for 72 hours. Optimal concentration and incubation time may vary and should be optimized by each laboratory. 

A: This kit is designed to measure PARP activity before and after apoptosis. It can be used for the screening of candidate PARP-1 inhibitors and determination of IC50 values.

A: The wash buffer containing PBS-Triton results in foaming which is difficult to remove without subsequent PBS washes. Signal intensity and reproducibility are also decreased if PBS-Triton wash is not followed up with a PBS wash.