HTRA2/Omi Antibody (ST54-05)
Novus Biologicals | Catalog # NBP2-67543
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ST54-05 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within Human HTRA2/Omi aa 800-188 / 458. (SwissProt: O43464 Human; SwissProt: Q9JIY5 Mouse; Unigene: 107325 Rat)
Localization
Mitochondrion intermembrane space, Mitochondrion membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HTRA2/Omi Antibody (ST54-05)
Western Blot: HTRA2/Omi Antibody (ST54-05) [NBP2-67543]
Western Blot: HTRA2/Omi Antibody (ST54-05) [NBP2-67543] - Analysis of HtrA2 on 293 cell lysates using anti-HtrA2 antibody at 1/1,000 dilution.Immunohistochemistry-Paraffin: HTRA2/Omi Antibody (ST54-05) [NBP2-67543]
Immunohistochemistry-Paraffin: HTRA2/Omi Antibody (ST54-05) [NBP2-67543] - Analysis of paraffin-embedded mouse liver tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: HTRA2/Omi Antibody (ST54-05) [NBP2-67543]
Immunohistochemistry-Paraffin: HTRA2/Omi Antibody (ST54-05) [NBP2-67543] - Analysis of paraffin-embedded human colon cancer tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: HTRA2/Omi Antibody (ST54-05) [NBP2-67543]
Immunohistochemistry-Paraffin: HTRA2/Omi Antibody (ST54-05) [NBP2-67543] - Analysis of paraffin-embedded human liver tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.Applications for HTRA2/Omi Antibody (ST54-05)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HTRA2/Omi
Long Name
High Temperature Requirement Protein-2
Alternate Names
Omi, PRSS25
Gene Symbol
HTRA2
Additional HTRA2/Omi Products
Product Documents for HTRA2/Omi Antibody (ST54-05)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HTRA2/Omi Antibody (ST54-05)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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