Human Adiponutrin/PNPLA3 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5208U Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5208U-100UG

Save 15% on Select RUO Reagents. See Details.

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Adiponutrin/PNPLA3 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Adiponutrin/PNPLA3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Adiponutrin/PNPLA3
Arg160-Arg349
Accession # Q9NST1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Adiponutrin/PNPLA3

Human Adiponutrin (ADPN; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53 kDa member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481 amino acids (aa) in length. It contains one patatin domain (aa 10‑179) that is associated with in vitro enzyme activity and a transmembrane segment between aa 42‑62. There appear to be two splice variants. One shows a four aa deletion of aa 59‑62 and a second shows an alternate start site at Met379. Over aa 160‑349, human ADPN shares 61% aa identity with mouse Adiponutrin.

Long Name

Patanin-like Phospholipase Domain-containing Protein 3

Entrez Gene IDs

80339 (Human); 362972 (Rat)

Alternate Names

Acylglycerol O-acyltransferase; Adiponutrin; ADPN; ADPNdJ796I17.1; C22orf20; Calcium-independent phospholipase A2-epsilon; chromosome 22 open reading frame 20; EC 2.3.1.-; EC 2.7.7.56; EC 3.1.1.3; EC 4.2.3.4; FLJ22012; iPLA(2)epsilon; iPLA2-epsilon; patatin-like phospholipase domain containing 3; patatin-like phospholipase domain-containing protein 3; PNPLA3