Human APRIL/TNFSF13 Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Saliva (25 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva)
Specificity
natural and recombinant human APRIL.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human APRIL/TNFSF13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human APRIL in cell culture supernates, serum, platelet-poor plasma, and saliva. It contains HEK293-expressed recombinant human APRIL and antibodies raised against the recombinant factor. Results obtained using natural human APRIL showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human APRIL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean (ng/mL) |
1.42 |
2.84 |
6.24 |
1.48 |
3.06 |
6.09 |
| Standard Deviation |
0.075 |
0.109 |
0.265 |
0.126 |
0.198 |
0.401 |
| CV% |
5.3 |
3.8 |
4.2 |
8.5 |
6.5 |
6.6 |
Recovery
The recovery of human APRIL spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
99 |
96-105 |
| Platelet-poor EDTA Plasma (n=4) |
102 |
92-111 |
| Platelet-poor Heparin Plasma (n=4) |
104 |
100-109 |
| Serum (n=4) |
102 |
94-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human APRIL were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Product Datasheets
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Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: APRIL/TNFSF13
A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice.
Long Name:
A Proliferation-inducing Ligand
Entrez Gene IDs:
8741 (Human); 69583 (Mouse); 287437 (Rat); 102126587 (Cynomolgus Monkey)
Alternate Names:
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2
