Human B7-H3 Alexa Fluor® 405-conjugated Antibody

FAB1027V Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1027V-025

Bulk Orders

Human B7-H3 Alexa Fluor® 405-conjugated Antibody in Flow Cytometry

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-H3 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 185504

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7‑H3
Leu29-Pro245
Accession # NP_079516

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

View Larger

Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1027V, filled histogram) or isotype control antibody (Catalog # IC002V, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H3

B7-H3 is a member of the B7 family of immune co-stimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

Long Name

B7 Homolog 3

Entrez Gene IDs

80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)

Alternate Names

B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule