Home / B7-H3 / Human B7-H3 Alexa Fluor® 405-conjugated Antibody

Human B7-H3 Alexa Fluor® 405-conjugated Antibody

FAB1027V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1027V-025
Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry.
1 Image
Product Details
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-H3 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 185504
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7‑H3
Leu29-Pro245
Accession # NP_079516
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry. View Larger

Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human B7‑H3 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1027V, filled histogram) or isotype control antibody (Catalog # IC002V, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H3

B7-H3 is a member of the B7 family of immune co-stimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

Long Name
B7 Homolog 3
Entrez Gene IDs
80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule

Product Datasheets

Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

IC002V
Ctrl

Staining Reagents

Flow Cytometry Human Lyse Buffer (10X)

FC002
1 Citations
1 Review
Flow

Flow Cytometry Staining Buffer (1X)

FC001
12 Citations
2 Reviews
Flow

Flow Cytometry Mouse Lyse Buffer (10X)

FC003
5 Citations
1 Review
Flow

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