Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 185504
View all B7-H3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7‑H3
Leu29-Pro245
Accession # NP_079516

Specificity

Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human B7‑H3 APC‑conjugated Antibody

Detection of B7-H3 antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 APC-conjugated Monoclonal Antibody (Catalog # FAB1027A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human B7‑H3 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: PC‑3 human prostate cancer cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H3

B7-H3 is a member of the B7 family of immune costimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Entrez Gene IDs

80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)

Gene Symbol

CD276

UniProt

NP_079516

Additional B7-H3 Products

Product Documents for Human B7‑H3 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human B7‑H3 APC‑conjugated Antibody

For research use only

Citations for Human B7‑H3 APC‑conjugated Antibody

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