Human BAI1 Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # AF4969AFP647

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Polyclonal Sheep IgG
View all BAI1 Primary Antibodies »

Product Specifications

Specificity

Detects human BAI1 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human BAI3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human BAI1 Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alpha V beta 5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

References

  1. Nishimori, H. et al. (1997) Oncogene 15:2145.
  2. Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.
  3. Mori, K. et al. (2002) Neurosci. Res. 43:69.
  4. Kaur, B. et al. (2003) Am. J. Pathol. 162:19.
  5. Park, D. et al. (2007) Nature 450:430.
  6. Duda, D.G. et al. (2002) Br. J. Cancer 86:490.
  7. Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.
  8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.
  9. Kaur, B. et al. (2005) Oncogene 24:3632.
  10. Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.
  11. Kaur, B. et al. (2009) Cancer Res. 69:1212.
  12. Kang, X. et al. (2006) Cancer Gene Ther. 13:385.
  13. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Entrez Gene IDs

575 (Human); 107831 (Mouse); 362931 (Rat)

Gene Symbol

ADGRB1

UniProt

O14514

Additional BAI1 Products

Product Documents for Human BAI1 Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BAI1 Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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