Human BTN1A1/Butyrophilin Alexa Fluor® 750-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB84671S-100UG
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Human BTN1A1/Butyrophilin Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTN1A1/Butyrophilin in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2151B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTN1A1/Butyrophilin
Ala27-Arg242
Accession # Q13410
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line Transfected with Human BTN1A1 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTN1A1/Butyrophilin

Butyrophilin 1A1 (also called BTN1A1), a 55kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 494 amino acids (aa) long and is composed of an extracellular domain (ECD) (aa 27-242), a transmembrane domain and a cytoplasmic tail (aa 270-526) which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma δ T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4+ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of human BTN1A1 shares 68% aa sequence identity with both mouse and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.

References
  1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  2. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
  3. Vorback, C. et al. (2002) Genes Dev. 16:3223.
  4. Ogg, S.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10084.
  5. Robenek, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10385.
  6. Sandstrom, A. et al. (2014) Immunity 40:490.
  7. Arnet, H.A. and Viney, J.L. (2014) Nat.Rev.Immunol.14:559.
  8. Smith, I.A. et al. (2010) J.Immunol. 184:3514.
  9. Mana, P. et al. (2004) J.Immunol. 16:489.
Entrez Gene IDs
696 (Human); 12231 (Mouse); 306956 (Rat)
Alternate Names
BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1); BTN; BTN1A1; butyrophilin subfamily 1 member A1; butyrophilin, subfamily 1, member A1

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