Human BTN1A1/Butyrophilin Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human BTN1A1/Butyrophilin in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 2151B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BTN1A1/Butyrophilin
    Ala27-Arg242
    Accession # Q13410
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunocytochemistry
    0.3-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BTN1A1 in HEK293 Human Cell Line Transfected with Human BTN1A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human BTN1A1 or (B) irrelelvant transfectants and eGFP was stained with Rabbit Anti-Human BTN1A1 Monoclonal Antibody (Catalog # MAB84671) followed by APC-conjugated Goat-anti Rabbit IgG secondary antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
BTN1A1/Butyrophilin in HEK293 Human Cell Line. BTN1A1/Butyrophilin was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human BTN1A1/Butyrophilin Polyclonal Antibody (Catalog # MAB84671) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BTN1A1/Butyrophilin

Butyrophilin 1A1 (also called BTN1A1), a 55kDa type I transmembrane glycoprotein, is a member of the Ig superfamily. BTN1A1 is 494 amino acids (aa) long and is composed of an extracellular domain (ECD) (aa 27-242), a transmembrane domain and a cytoplasmic tail (aa 270-526) which contains the B30.2 domain. The BTN1A1 ECD displays two predicted IgV and IgC domains as do B7 and Skint proteins which interact with other Ig superfamily members (1).The B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) (2). This interaction stabilizes the association of XOR with the milk fat globule membrane and appears to be essential in the control of milk fat globule secretion (3, 4, 5). Binding to XOR is conserved among BTN1A1 orthologs, but is not shared by BTN2A1 or BTN3A1 (2). The B30.2 domain of butyrophilins is also described as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations. B30.2 binding to pAg induces a cascade of events leading to the activation of gamma δ T cells (6). In vitro, BTN1A1 has an inhibitory effect on CD4+ T cell proliferation, and in addition reduces expression of cytokines associated with T cell activation such as IL-2 and IFN-gamma (7, 8). Furthermore, in vivo, BTN1A1 has a protective effect against the development of experimental autoimmune encephalomyelitis (EAE) (9). The ECD of human BTN1A1 shares 68% aa sequence identity with both mouse and rat BTN1A1. Because butyrophilins are structurally related to B7 proteins and are functionally implicated in immune regulation, they may represent an emerging family of co-stimulatory/inhibitory molecules.

  • References:
    1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
    2. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
    3. Vorback, C. et al. (2002) Genes Dev. 16:3223.
    4. Ogg, S.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10084.
    5. Robenek, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10385.
    6. Sandstrom, A. et al. (2014) Immunity 40:490.
    7. Arnet, H.A. and Viney, J.L. (2014) Nat.Rev.Immunol.14:559.
    8. Smith, I.A. et al. (2010) J.Immunol. 184:3514.
    9. Mana, P. et al. (2004) J.Immunol. 16:489.
  • Entrez Gene IDs:
    696 (Human); 12231 (Mouse); 306956 (Rat)
  • Alternate Names:
    BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1); BTN; BTN1A1; butyrophilin subfamily 1 member A1; butyrophilin, subfamily 1, member A1
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