Human Calreticulin PE‑conjugated Antibody

R&D Systems | Catalog # IC3898P

R&D Systems
Discontinued Product
IC3898P has been discontinued. View all Calreticulin products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 326203
View all Calreticulin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Calreticulin
Met1-Asn180
Accession # P27797

Specificity

Detects human Calreticulin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Calreticulin PE‑conjugated Antibody

Detection of Calreticulin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin PE-conjugated Monoclonal Antibody (Catalog # IC3898P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Calreticulin PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Calreticulin

Human Calreticulin is a 55‑60 kDa, 400 amino acid (aa), variably glycosylated intra- and extracellular Ca++-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. The Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.

Alternate Names

Autoantigen Ro, CALR, Calregulin, cC1qR, CRT, SSA, Vasostatin

Entrez Gene IDs

811 (Human); 12317 (Mouse); 64202 (Rat)

Gene Symbol

CALR

UniProt

P27797 (Human)

Additional Calreticulin Products

Product Documents for Human Calreticulin PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Calreticulin PE‑conjugated Antibody

For research use only

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