Mouse IgG2B PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC0041P

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Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (40)
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Reviews (1)

Mouse IgG2B PE-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2B Clone # 133303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody (Catalog # IC0041P, open histogram).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

Product Datasheets

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Citations for Mouse IgG2B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Interleukin-6 Signaling in Triple Negative Breast Cancer Cells Elicits the Annexin A1/Formyl Peptide Receptor 1 Axis and Affects the Tumor Microenvironment
    Authors: L Vecchi, STS Mota, MAP Zóia, IC Martins, JB de Souza, TG Santos, AO Beserra, VP de Andrade, LR Goulart, TG Araújo
    Cells, 2022;11(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  2. Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
    Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
    Cellular Signalling, 2022;96(0):110360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD200-CD200R immune checkpoint engagement regulates ILC2 effector function and ameliorates lung inflammation in asthma
    Authors: P Shafiei-Ja, DG Helou, BP Hurrell, E Howard, C Quach, JD Painter, L Galle-Treg, M Li, YE Loh, O Akbari
    Nature Communications, 2021;12(1):2526.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Mev-stealth: A cd46-specific oncolytic measles virus resistant to neutralization by measles-immune human serum
    Authors: MÁ Muñoz-Alía, RA Nace, A Tischer, L Zhang, ES Bah, M Auton, SJ Russell
    PloS Pathogens, 2021;17(2):e1009283.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. MicroRNA-222 Regulates Melanoma Plasticity
    Authors: MC Lionetti, F Cola, O Chepizhko, MR Fumagalli, F Font-Clos, R Ravasio, S Minucci, P Canzano, M Camera, G Tiana, S Zapperi, CAM Porta
    J Clin Med, 2020;9(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Annexin A1 promotes the nuclear localization of the epidermal growth factor receptor in castration-resistant prostate cancer
    Authors: STS Mota, L Vecchi, DA Alves, AO Cordeiro, GS Guimarães, E Campos-Fer, YCP Maia, BC Dornelas, SM Bezerra, VP de Andrade, LR Goulart, TG Araújo
    Int. J. Biochem. Cell Biol., 2020;127(0):105838.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  7. A novel S1P1 modulator IMMH002 ameliorates psoriasis in multiple animal models
    Authors: J Jin, N Xue, Y Liu, R Fu, M Wang, M Ji, F Lai, J Hu, X Wang, Q Xiao, X Zhang, D Yin, L Bai, X Chen, S Rao
    Acta Pharm Sin B, 2020;10(2):276-288.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. DR3 stimulation of adipose resident ILC2s ameliorates type 2 diabetes mellitus
    Authors: P Shafiei-Ja, BP Hurrell, L Galle-Treg, DG Helou, E Howard, J Painter, R Lo, G Lewis, P Soroosh, O Akbari
    Nat Commun, 2020;11(1):4718.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK
    Authors: J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su
    Arthritis Research & Therapy, 2020;22(1):221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. MicroRNA-222 Regulates Melanoma Plasticity
    Authors: MC Lionetti, F Cola, O Chepizhko, MR Fumagalli, F Font-Clos, R Ravasio, S Minucci, P Canzano, M Camera, G Tiana, S Zapperi, CAM Porta
    J Clin Med, 2020;9(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. New CXCR4 Antagonist Peptide R (Pep R) Improves Standard Therapy in Colorectal Cancer
    Authors: C D'Alterio, A Zannetti, AM Trotta, C Ieranò, M Napolitano, G Rea, A Greco, P Maiolino, S Albanese, G Scognamigl, F Tatangelo, S Tafuto, L Portella, S Santagata, G Nasti, A Ottaiano, R Pacelli, P Delrio, G Botti, S Scala
    Cancers (Basel), 2020;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Elevated Expression of C-Type Lectin Domain Family 5-Member A (CLEC5A) and Its Relation to Inflammatory Parameters and Disease Course in Adult-Onset Still's Disease
    Authors: PK Chen, SL Hsieh, JL Lan, CC Lin, SH Chang, DY Chen
    J Immunol Res, 2020;2020(0):9473497.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  13. Next-generation hypomethylating agent SGI-110 primes acute myeloid leukemia cells to IAP antagonist by activating extrinsic and intrinsic apoptosis pathways
    Authors: J Dittmann, T Haydn, P Metzger, GA Ward, M Boerries, M Vogler, S Fulda
    Cell Death Differ., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. TIM-1 As a Signal Receptor Triggers Dengue Virus-Induced Autophagy
    Authors: LW Chu, CJ Yang, KJ Peng, PL Chen, SJ Wang, YH Ping
    Int J Mol Sci, 2019;20(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Influence of endocrine therapy on the ratio of androgen receptor (AR) to estrogen receptor (ER) positive circulating epithelial tumor cells (CETCs) in breast cancer
    Authors: M Pizon, D Lux, U Pachmann, K Pachmann, D Schott
    J Transl Med, 2018;16(1):356.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines
    Authors: I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters
    Mol Oncol, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Apoptosis-sensitizing activity of birinapant in head and neck squamous cell carcinoma cell lines
    Authors: RC Brands, MJJ Scheurer, S Hartmann, A Seher, AC Kübler, UDA Müller-Ric
    Oncol Lett, 2018;15(3):4010-4016.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. IL-7 Restores T Lymphocyte Immunometabolic Failure in Septic Shock Patients through mTOR Activation
    Authors: F Venet, J Demaret, BJ Blaise, C Rouget, T Girardot, E Idealisoa, T Rimmelé, F Mallet, A Lepape, J Textoris, G Monneret
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
    Authors: M Dejosez, TP Zwaka
    Stem Cell Res, 2017;26(0):44-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity
    Authors: M Fischer, JP Müller, B Spies-Weis, C Gräfe, O Kurzai, K Hünniger, A Hochhaus, S Scholl, U Schnetzke
    Eur. J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  21. The DRF motif of CXCR6 as chemokine receptor adaptation to adhesion
    Authors: A Koenen, A Babendreye, J Schumacher, T Pasqualon, N Schwarz, A Seifert, X Deupi, A Ludwig, D Dreymuelle
    PLoS ONE, 2017;12(3):e0173486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages
    Authors: OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura
    Redox Biol, 2017;15(0):1-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity
    Authors: Lars Fugger
    Sci Transl Med, 2016;8(363):363ra149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  25. The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
    Authors: Warat M, Sadowski T, Szliszka E, Krol W, Czuba Z
    Molecules, 2015;20(1):900-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  26. Aberrant CCR4 expression is involved in tumor invasion of lymph node-negative human gastric cancer.
    Authors: Yang, Yongmei, Du, Lutao, Yang, Xiaoyun, Qu, Ailin, Zhang, Xin, Zhou, Chengjun, Wang, Chuanxin
    PLoS ONE, 2015;10(3):e0120059.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Endothelial cell-derived chemerin promotes dendritic cell transmigration.
    Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
    J Immunol, 2014;192(5):2366-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  28. Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages.
    Authors: Warat M, Szliszka E, Korzonek-Szlacheta I, Krol W, Czuba Z
    Int J Mol Sci, 2014;15(7):11510-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  29. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014;192(11):5332-42.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  30. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014;9(6):e99056.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
    Authors: Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, Wang G, Walser T, Krysan K, Sharma S, Dubinett S, Lee J
    J Exp Clin Cancer Res, 2013;32(0):97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  32. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012;7(12):e50589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  33. Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
    Authors: Noyce RS, Bondre DG, Ha MN
    PLoS Pathog., 2011;7(8):e1002240.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Control
  34. The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells.
    Authors: Szliszka E, Helewski KJ, Mizgala E, Krol W
    Int. J. Oncol., 2011;39(4):771-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  35. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010;116(15):2793-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  36. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro.
    Authors: Zhang Y, Fisher N, Newey SE
    Stem Cells Dev., 2009;18(2):359-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  37. Epigenetic silencing of death receptor 4 mediates tumor necrosis factor-related apoptosis-inducing ligand resistance in gliomas.
    Authors: Elias A, Siegelin MD, Steinmuller A, von Deimling A, Lass U, Korn B, Mueller W
    Clin. Cancer Res., 2009;15(17):5457-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  38. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009;127(4):549-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  40. Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2.
    Authors: Dai CL, Tiwari AK, Wu CP, Su XD, Wang SR, Liu DG, Ashby CR, Huang Y, Robey RW, Liang YJ, Chen LM, Shi CJ, Ambudkar SV, Chen ZS, Fu LW
    Cancer Res., 2008;68(19):7905-14.

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Mouse IgG2B PE-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Blood-derived monocytes Species: Human