Human CCL2/MCP-1 XL Luminex® Performance Assay

Name: Human CCL2/MCP-1 XL Luminex® Performance Assay
Brand: R&D Systems
SKU: LUXLM279
Price: 210 USD
Availability: InStock

R&D Systems | Catalog # LUXLM279

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Product Documents

Luminex High Performance Assays

Human CCL2/MCP-1 XL Luminex® Performance Assay is specifically designed for multiplex detection of human CCL2/JE/MCP-1 with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.

This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.

Build Now

Build Your Assay
Product Features
Performance Validation
Kit Contents
Precision And Accuracy
Which Luminex Assay Is Right For Me
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Panel

Human XL Cytokine Panel

Assay Type

Magnetic bead-based multiplex assay for the Luminex® platform

Format

1 x 96-well microplate and magnetic antibody-coated beads

Species

Human

Analytes Detected

CCL2/JE/MCP-1

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

View all CCL2/JE/MCP-1 Luminex Assays »

Build Your Assay

Make Your Selections Below to Configure Your Custom Luminex® Assay

Configured Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If overlapping bead regions occur in analyte selections, send your Luminex configuration code to our team for resolution.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on sample dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human CCL2/JE/MCP-1 with a panel of related analytes in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate
Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*

*Please allow additional time for international orders.

Performance Validation

R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Human XL validation data.

Base Kit Contents

Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**
High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Bead Set Contents

User-mixed antibody-coated Magnetic beads in individual vials

Recommended Sample Types: Cell culture supernates, serum, EDTA plasma, and heparin plasma

Components: Human CCL2/MCP-1 Magnetic Microparticles (Part 898814) is supplied as a 100X concentrated stock (0.075 mL) with preservatives. Other Supplies Required: Magnetic Luminex® Performance Assay Human XL Discovery Base Kit (R&D Systems®, Catalog # LUXLM000).

Sensitivity: The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the mean MFI of twenty zero standard replicates and calculating the corresponding concentration. Six assays were evaluated, and the MDD of human MCP-1 ranged from 0.312-2.00 pg/mL. The mean MDD was 0.595 pg/mL.

Correlation: This assay has been correlated to the Quantikine® ELISA Kit.

Specificity: This assay recognizes natural and recombinant human MCP-1.

Bead Region: 15

Precision

	Intra-Assay Precision		Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Two samples of known concentration were tested in thirty separate assays to assess precision between assays.
Sample	1	2	1	2
n	20	20	30	30
Mean (pg/mL)	52.2	529	54.4	554
Standard deviation	0.73	11.9	4.56	23.0
CV (%)	39.8	24.9	37.5	16.6

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human MCP-1 were evaluated for recovery.
Cell culture supernate	134	114-146
Serum	99.0	46.8-128
EDTA plasma	100	49.3-136
Heparin plasma	99.9	49.3-122
Plaelet-poor EDTA	106	74.1-164
Platelet-poor Heparin	100	66.7-143

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma	Platelet-poor EDTA	Platelet-poor Heparin
Samples containing and/or spiked with human MCP-1 were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	87.1	108	114	116	113	109
1:2	Range (%)	78.3-94.0	96.1-139	101-135	100-140	108-117	104-117
1:4	Average % of Expected	85.2	109	116	116	115	113
1:4	Range (%)	76.8-92.3	92.0-159	93.9-159	94.3-159	111-122	104-120
1:8	Average % of Expected	83.6	118	122	121	120	114
1:8	Range (%)	73.6-89.7	93.9-187	95.8-177	92.2-179	112-131	107-130
1:16	Average % of Expected	84.0	114	118	117	118	109
1:16	Range (%)	74.8-89.4	89.4-184	91.4-177	88.3-180	110-131	102-131

Which Luminex Assay is Right for Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.

High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.

Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.

Additional Information About Luminex Assays

All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.

Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.

Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.

Scientific Data Images for Human CCL2/MCP-1 XL Luminex® Performance Assay

MCP-1 XL Cytokine Luminex Panel Standard Curves

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4⁺ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.