Human CCL23/MPIF‑1 Antibody

R&D Systems | Catalog # MAB371

R&D Systems
Discontinued Product
MAB371 has been discontinued. An alternative/replacement product is available: AF371. View all CCL23/MPIF-1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Neutralization, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 69512
View all CCL23/MPIF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL23/MPIF-1
Arg22-Asn120
Accession # P55773

Specificity

Detects human CCL23/MPIF‑1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL23/MPIF‑1 Antibody

Chemotaxis Induced by CCL23/MPIF‑1 and Neutral-ization by Human CCL23/MPIF‑1 Antibody.

Chemotaxis Induced by CCL23/MPIF‑1 and Neutral-ization by Human CCL23/MPIF‑1 Antibody.

Recombinant Human CCL23/MPIF-1 (Catalog # 371-MP) chemoattracts the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF-1 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL23/MPIF-1 Monoclonal Antibody (Catalog # MAB371). The ND50 is typically 2-6 µg/mL.

Applications for Human CCL23/MPIF‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP)

Neutralization

Measured by its ability to neutralize CCL23/MPIF‑1-induced chemotaxis in the THP‑1 human acute monocytic leukemia cell line. Howell, D. N. et al., 1985, J. Immunol. 134:971. The Neutralization Dose (ND50) is typically 2‑6 µg/mL in the presence of 1 µg/mL Recombinant Human CCL23/MPIF‑1.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL23/MPIF-1

CCL23, also known as Myeloid Progenitor Inhibitory Factor-1 (MPIF-1), is a CC chemokine that is produced by some myeloid cell lines and monocyte-derived dendritic cells. It binds CCR1 and inhibits the differentiation of progenitor cells that give rise to granulocytes and macrophages. CCL23 is also chemotactic for resting T cells, neutrophils, monocytes and dendritic cells.

Alternate Names

CCL23, MPIF-1, MPIF1

Entrez Gene IDs

6368 (Human)

Gene Symbol

CCL23

UniProt

P55773

Additional CCL23/MPIF-1 Products

Product Documents for Human CCL23/MPIF‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL23/MPIF‑1 Antibody

For research use only

Citations for Human CCL23/MPIF‑1 Antibody

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