Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin
Measured by its ability to neutralize CCL23/MPIF‑1-induced chemotaxis in the THP‑1 human acute monocytic leukemia cell line. Howell, D. N. et al., 1985, J. Immunol. 134:971. The Neutralization Dose (ND50) is typically 2‑6 µg/mL in the presence of 1 µg/mL Recombinant Human CCL23/MPIF‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL23/MPIF‑1 and Neutralization by Human CCL23/MPIF‑1 Antibody. Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP) chemoattracts the THP‑1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF‑1 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL23/MPIF‑1 Monoclonal Antibody (Catalog # MAB371). The ND50 is typically 2-6 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL23, also known as Myeloid Progenitor Inhibitory Factor-1 (MPIF-1),
is a CC chemokine that is produced by some myeloid cell lines and
monocyte-derived dendritic cells. It binds CCR1 and inhibits the
differentiation of progenitor cells that give rise to granulocytes and
macrophages. CCL23 is also chemotactic for resting T cells,
neutrophils, monocytes and dendritic cells.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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