Human CCL23/MPIF-1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL23/MPIF‑1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 69512
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CCL23/MPIF-1
    Arg22-Asn120
    Accession # P55773
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin
  • Neutralization
    Measured by its ability to neutralize CCL23/MPIF‑1-induced chemotaxis in the THP‑1 human acute monocytic leukemia cell line. Howell, D. N. et al., 1985, J. Immunol. 134:971. The Neutralization Dose (ND50) is typically 2‑6 µg/mL in the presence of 1 µg/mL Recombinant Human CCL23/MPIF‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL23/MPIF‑1 and Neutral­ization by Human CCL23/MPIF‑1 Antibody. Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP) chemoattracts the THP‑1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF‑1 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL23/MPIF‑1 Monoclonal Antibody (Catalog # MAB371). The ND50 is typically 2-6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL23/MPIF-1
CCL23, also known as Myeloid Progenitor Inhibitory Factor-1 (MPIF-1), is a CC chemokine that is produced by some myeloid cell lines and monocyte-derived dendritic cells. It binds CCR1 and inhibits the differentiation of progenitor cells that give rise to granulocytes and macrophages. CCL23 is also chemotactic for resting T cells, neutrophils, monocytes and dendritic cells.
  • Entrez Gene IDs:
    6368 (Human)
  • Alternate Names:
    CCL23; CCL23/MPIF-1; MPIF1; MPIF-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. CCL23 expression is induced by IL-4 in a STAT6-dependent fashion.
    Authors: Novak H, Muller A, Harrer N, Gunther C, Carballido JM, Woisetschlager M
    J. Immunol., 2007;178(7):4335-41.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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