Human CCL23/MPIF-1 Antibody

Catalog # Availability Size / Price Qty
MAB371
MAB371-SP
Human CCL23/MPIF-1 Antibody in Data
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Citations (1)
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Human CCL23/MPIF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL23/MPIF‑1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 69512
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL23/MPIF-1
Arg22-Asn120
Accession # P55773
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin
Neutralization
Measured by its ability to neutralize CCL23/MPIF‑1-induced chemotaxis in the THP‑1 human acute monocytic leukemia cell line. Howell, D. N. et al., 1985, J. Immunol. 134:971. The Neutralization Dose (ND50) is typically 2‑6 µg/mL in the presence of 1 µg/mL Recombinant Human CCL23/MPIF‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by CCL23/MPIF‑1 and Neutral-ization by Human CCL23/MPIF‑1 Antibody. View Larger

Chemotaxis Induced by CCL23/MPIF‑1 and Neutral-ization by Human CCL23/MPIF‑1 Antibody. Recombinant Human CCL23/MPIF-1 (Catalog # 371-MP) chemoattracts the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF-1 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL23/MPIF-1 Monoclonal Antibody (Catalog # MAB371). The ND50is typically 2-6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL23/MPIF-1

CCL23, also known as Myeloid Progenitor Inhibitory Factor-1 (MPIF-1), is a CC chemokine that is produced by some myeloid cell lines and monocyte-derived dendritic cells. It binds CCR1 and inhibits the differentiation of progenitor cells that give rise to granulocytes and macrophages. CCL23 is also chemotactic for resting T cells, neutrophils, monocytes and dendritic cells.

Entrez Gene IDs
6368 (Human)
Alternate Names
CCL23; CCL23/MPIF-1; MPIF1; MPIF-1

Product Datasheets

Citation for Human CCL23/MPIF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CCL23 expression is induced by IL-4 in a STAT6-dependent fashion.
    Authors: Novak H, Muller A, Harrer N, Gunther C, Carballido JM, Woisetschlager M
    J. Immunol., 2007;178(7):4335-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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