CCL26, also known as Eotaxin-3 and SCYA26, is a small cytokine that is a member of the CC chemokine family. It is only 94 amino acids (aa) in length, with aa 1-23 serving as a putative signal peptide. Human CCL26/Eotaxin-3 has a predicted molecular weight of approximately 11 kDa, and the mature protein only shares 27% aa sequence identity with the mouse ortholog. CCL26/Eotaxin-3 is constitutively expressed at low levels in various tissues including in the heart and ovary. Its expression in upregulated in vascular endothelial cells by IL-13 and IL-4. CCL26/Eotaxin-3 is a selective agonist for CCR3, as serves as a chemoattractant for cells that express CCR3, such as eosinophils, basophils, and Th2 cells. It has also been shown to act as an antagonist for the receptors CCR1 and CCR5. CCL26/Eotaxin-3 plays a role in allergic reactions.
Human CCL26/Eotaxin-3 Quantikine ELISA Kit
R&D Systems | Catalog # DCC260
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
Product Specifications
Assay Type
Format
Measurement
Detection Method
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 42.4 | 114 | 291 | 50.3 | 126 | 293 |
| Standard Deviation | 2.37 | 4.23 | 9.62 | 3.78 | 5.53 | 18.8 |
| CV% | 5.6 | 3.7 | 3.3 | 7.5 | 4.4 | 6.4 |
Recovery for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
The recovery of human Eotaxin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 92-112 |
| EDTA Plasma (n=5) | 92 | 85-101 |
| Heparin Plasma (n=5) | 92 | 86-100 |
| Serum (n=5) | 91 | 86-98 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of recombinant human or natural Eotaxin-3 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
Human Eotaxin-3 ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL26/Eotaxin-3
Additional CCL26/Eotaxin-3 Products
Product Documents for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
For research use only
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Citations for Human CCL26/Eotaxin-3 Quantikine ELISA Kit
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