Home » CD35 » Human CD35 Alexa Fluor® 700-conjugated Antibody
Human CD35 Alexa Fluor® 700-conjugated Antibody
Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed.
Monoclonal Mouse IgG1 Clone # 594708
Mouse myeloma cell line NS0-derived recombinant human CD35 Gln42-Asp1971 (His1208Arg) Accession # CAA68755
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700
0.25-1 µg/106 cells
Human peripheral blood lymphocytes (PBMC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
CD35, also known as complement receptor 1 (CR1), is a 220-300 kDa N-glycosylated member of the RCA (regulators of complement activation) family of proteins. CD35 binds and internalizes particles and immune complexes that are opsonized with MBL or complement components C3b, C3i, C4b, or C1q. CD35 additionally protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases. The extracellular domain (ECD) of human CD35 contains 30 tandem SCR/SUSHI repeats. A soluble form of the CD35 ECD circulates in the serum. A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35. Cell surface CD35 is widely expressed on hematopoietic cells.
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