Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human CD40 Ligand/TNFSF5 with a panel of
related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of
material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Base Kit Contents
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
- High and low controls with Control Mean Value Card
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
Recommended Sample Types: Cell culture supernates, serum, platelet-poor plasma
Components: Human CD40 Ligand/TNFSF5 Magnetic Microparticles (Part 898852) is supplied as a 100X concentrated stock (0.075 mL) with preservatives.
Other Supplies Required: Magnetic Luminex® Performance Assay Human XL Discovery Base Kit (R&D Systems®, Catalog # LUXLM000).
Sensitivity: The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the mean MFI of twenty zero standard replicates and calculating the corresponding concentration.
Six assays were evaluated, and the MDD of human CD40 Ligand ranged from 49.5-379 pg/mL. The mean MDD was 110 pg/mL.
Correlation: This assay has been correlated to the Quantikine ELISA Kit.
Bead Region: 25
Precision
|
Intra-assay Precision (precision within an assay) - Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (precision between assays) - Two samples of known concentration were tested in thirty separate assays to assess precision between assays.
|
| |
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
1 |
2 |
| n |
20 |
20 |
30 |
30 |
| Mean (pg/mL) |
5323 |
52,667 |
5450 |
52,704 |
| Standard deviation |
154 |
2455 |
766 |
3993 |
| CV (%) |
89.3 |
66.1 |
14.0 |
57.6 |
Recovery
|
Samples containing and/or spiked with human CD40 Ligand were evaluated for recovery.
|
| Sample Type |
Average % Recovery |
Range (%) |
| Cell culture supernate |
114 |
105-126 |
| Serum |
75 |
61-102 |
| EDTA plasma |
87 |
66-111 |
| Heparin plasma |
86 |
70-101 |
| Plaelet-poor EDTA |
78 |
56-94 |
| Platelet-poor Heparin |
79 |
60-101 |
Linearity
|
Samples containing and/or spiked with human CD40 Ligand were serially diluted to evaluate assay linearity.
|
| |
Cell culture supernate |
Serum |
EDTA plasma |
Heparin plasma |
Platelet-poor EDTA |
Platelet-poor Heparin |
| 1:2 |
Average % of Expected |
98 |
125 |
120 |
114 |
124 |
120 |
| Range (%) |
94-103 |
119-133 |
112-129 |
101-130 |
115-138 |
114-128 |
| 1:4 |
Average % of Expected |
93 |
128 |
118 |
110 |
134 |
130 |
|
| Range (%) |
91-96 |
122-136 |
111-125 |
98-124 |
116-151 |
116-157 |
| 1:8 |
Average % of Expected |
95 |
137 |
124 |
114 |
149 |
131 |
| Range (%) |
88-100 |
128-143 |
113-141 |
98-128 |
123-172 |
125-139 |
| 1:16 |
Average % of Expected |
97 |
131 |
118 |
106 |
143 |
123 |
| Range (%) |
94-100 |
123-141 |
108-139 |
97-121 |
121-173 |
116-130 |
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as
High Performance Assays or
Discovery Assays.
High Performance Assays are optimized and validated as complete panels with
a focused set of analytes. You can choose from Fixed Panels, Premixed options,
or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully
customized panels tailored to your research needs. With universal reagents
validated for compatibility across all analytes in the menu, they support
endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex
instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with
analyte-specific antibodies to enable multiplex quantitation. During the
assay, standards and samples are added to the wells, where target analytes
bind to the capture antibodies. A biotinylated detection antibody cocktail
is then introduced, followed by a Streptavidin-PE conjugate that generates a
fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal
intensity is measured using a Luminex instrument. These assays are designed to
deliver reliable and reproducible results with validated antibody pairs and
optimized protocols. They are well suited for applications that require
sensitivity, accuracy, and confidence in your data while enabling efficient
multiplexing to measure multiple targets simultaneously.
