Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 439424
View all CEACAM-6/CD66c Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM-6/CD66c
Lys35-Gly320
Accession # P40199

Specificity

Detects human CEACAM-6/CD66c in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -3, or -5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human CEACAM-6/CD66c Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human whole blood granulocytes and HEK293 human embryonic kidney cell line transfected with human CEACAM-6/CD66c and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CEACAM-6/CD66c

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific cross‑reacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
  3. Barnett, T. et al. (1988) Genomics 3:59.
  4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
  5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
  6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
  7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
  8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
  9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
  10. Kalina, T. et al. (2005) BMC Cancer 5:38.
  11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
  13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
  14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 6

Alternate Names

CD66c, CEACAM6, CEAL, NCA

Entrez Gene IDs

4680 (Human); 107126399 (Cynomolgus Monkey)

Gene Symbol

CEACAM6

UniProt

P40199

Additional CEACAM-6/CD66c Products

Product Documents for Human CEACAM-6/CD66c Alexa Fluor® 405-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CEACAM-6/CD66c Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Customer Reviews for Human CEACAM-6/CD66c Alexa Fluor® 405-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human CEACAM-6/CD66c Alexa Fluor® 405-conjugated Antibody and earn rewards!

Have you used Human CEACAM-6/CD66c Alexa Fluor® 405-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies