Human CEACAM-6/CD66c APC-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CEACAM-6/CD66c in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -3, or -5 is observed. By flow cytometry, no cross-reactivity with CEACAM-3, -4, -7, or -8 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 439424
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CEACAM-6/CD66c
    Lys35-Gly320
    Accession # P40199
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CEACAM‑6/CD66c in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM‑6/CD66c APC‑conjugated Monoclonal Antibody (Catalog # FAB3934A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CEACAM‑6/CD66c in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-6/CD66c or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM‑6/CD66c APC‑conjugated Monoclonal Antibody (Catalog # FAB3934A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CEACAM-6/CD66c

Carcinoembryonic Antigen-related Cell Adhesion Molecule 6 (CEACAM-6), previously called Nonspecific Cross‑reacting Antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbriae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).

  • References:
    1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
    2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
    3. Barnett, T. et al. (1988) Genomics 3:59.
    4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
    5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
    6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
    7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
    8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
    9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
    10. Kalina, T. et al. (2005) BMC Cancer 5:38.
    11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
    12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
    13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
    14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.
  • Long Name:
    Carcinoembryonic Antigen-related Cell Adhesion Molecule 6
  • Entrez Gene IDs:
    4680 (Human)
  • Alternate Names:
    carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen); carcinoembryonic antigen-related cell adhesion molecule 6; CD66c antigen; CD66c; CEACAM6; CEACAM-6; CEAL; NCA; NCACEAL; Non-specific crossreacting antigen; Normal cross-reacting antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. A CEACAM6-High Airway Neutrophil Phenotype and CEACAM6-High Epithelial Cells Are Features of Severe Asthma
    Authors: A Shikotra, DF Choy, S Siddiqui, G Arthur, DR Nagarkar, G Jia, AK Wright, CM Ohri, E Doran, CA Butler, B Hargadon, AR Abbas, J Jackman, LC Wu, LG Heaney, JR Arron, P Bradding
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis
    Authors: F Gebauer, D Wicklein, M Tachezy, T Grob, D Steinemann, G Manukjan, G Göhring, B Schlegelbe, H Maar, JR Izbicki, M Bockhorn, U Schumacher
    Anticancer Res, 2016;36(4):1507-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human CEACAM-6/CD66c APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information