Detects human CEACAM-6/CD66c in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -3, or -5 is observed. By flow cytometry, no cross-reactivity with CEACAM-3, -4, -7, or -8 is observed.
Monoclonal Mouse IgG2A Clone # 439424
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human CEACAM-6/CD66c Lys35-Gly320 Accession # P40199
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of CEACAM‑6/CD66c in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM‑6/CD66c APC‑conjugated Monoclonal Antibody (Catalog # FAB3934A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CEACAM‑6/CD66c in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-6/CD66c or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM‑6/CD66c APC‑conjugated Monoclonal Antibody (Catalog # FAB3934A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Carcinoembryonic Antigen-related Cell Adhesion Molecule 6 (CEACAM-6), previously called Nonspecific Cross‑reacting Antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbriae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).
Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
Barnett, T. et al. (1988) Genomics 3:59.
Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
Ilantzis, C. et al. (2002) Neoplasia 4:151.
Kalina, T. et al. (2005) BMC Cancer 5:38.
Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Have you used Human CEACAM-6/CD66c APC-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.