Human CEACAM-6/CD66c APC-conjugated Antibody Summary
Accession # P40199
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CEACAM‑6/CD66c in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM-6/CD66c APC-conjugated Monoclonal Antibody (Catalog # FAB3934A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CEACAM‑6/CD66c in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-6/CD66c or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM-6/CD66c APC-conjugated Monoclonal Antibody (Catalog # FAB3934A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Carcinoembryonic Antigen-related Cell Adhesion Molecule 6 (CEACAM-6), previously called Nonspecific Cross‑reacting Antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbriae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).
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Citations for Human CEACAM-6/CD66c APC-conjugated Antibody
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Citations: Showing 1 - 2
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A CEACAM6-High Airway Neutrophil Phenotype and CEACAM6-High Epithelial Cells Are Features of Severe Asthma
Authors: A Shikotra, DF Choy, S Siddiqui, G Arthur, DR Nagarkar, G Jia, AK Wright, CM Ohri, E Doran, CA Butler, B Hargadon, AR Abbas, J Jackman, LC Wu, LG Heaney, JR Arron, P Bradding
J. Immunol, 2017;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Establishment and Characterization of a Pair of Patient-derived Human Non-small Cell Lung Cancer Cell Lines from a Primary Tumor and Corresponding Lymph Node Metastasis
Authors: F Gebauer, D Wicklein, M Tachezy, T Grob, D Steinemann, G Manukjan, G Göhring, B Schlegelbe, H Maar, JR Izbicki, M Bockhorn, U Schumacher
Anticancer Res, 2016;36(4):1507-18.
Sample Types: Whole Cells
Applications: Flow Cytometry
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