Human CEACAM-6/CD66c Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CEACAM-6/CD66c in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -3, or -5 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 439424
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CEACAM-6/CD66c
    Lys35-Gly320
    Accession # P40199
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CEACAM‑6/CD66c (Catalog # 3934-CM)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CEACAM‑6/CD66c in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Mouse Anti‑Human CEACAM‑6/CD66c Monoclonal Antibody (Catalog # MAB3934, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Detection of CEACAM‑6/CD66c in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-6/CD66c or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM‑6/CD66c Monoclonal Antibody (Catalog # MAB3934) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-6/CD66c

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific cross‑reacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).

  • References:
    1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
    2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
    3. Barnett, T. et al. (1988) Genomics 3:59.
    4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
    5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
    6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
    7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
    8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
    9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
    10. Kalina, T. et al. (2005) BMC Cancer 5:38.
    11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
    12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
    13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
    14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.
  • Long Name:
    Carcinoembryonic Antigen-related Cell Adhesion Molecule 6
  • Entrez Gene IDs:
    4680 (Human)
  • Alternate Names:
    carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen); carcinoembryonic antigen-related cell adhesion molecule 6; CD66c antigen; CD66c; CEACAM6; CEACAM-6; CEAL; NCA; NCACEAL; Non-specific crossreacting antigen; Normal cross-reacting antigen
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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