Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 439424
View all CEACAM-6/CD66c Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CEACAM-6/CD66c
Lys35-Gly320
Accession # P40199

Specificity

Detects human CEACAM-6/CD66c in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEACAM-1, -3, or -5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CEACAM‑6/CD66c Antibody

Detection of CEACAM‑6/CD66c antibody in Human Granulocytes antibody by Flow Cytometry.

Detection of CEACAM‑6/CD66c in Human Granulocytes by Flow Cytometry.

Human whole blood granulocytes were stained with Mouse Anti-Human CEACAM-6/CD66c Monoclonal Antibody (Catalog # MAB3934, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Detection of CEACAM‑6/CD66c antibody in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP antibody by Flow Cytometry.

Detection of CEACAM‑6/CD66c in HEK293 Human Cell Line Transfected with Human CEACAM-6/CD66c and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human CEACAM-6/CD66c or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM-6/CD66c Monoclonal Antibody (Catalog # MAB3934) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Detection of CEACAM-6/CD66c by Flow Cytometry

Detection of CEACAM-6/CD66c by Flow Cytometry

SSc serum induced CEACAM6 expression on classical monocytes. CD14+16- monocytes were isolated from a healthy donor and were treated with serum from HCs (n=7) and patients with systemic sclerosis (n=14) for 15 h. The proportion of CEACAM-positive classical monocytes from patients with SSc who provided serum was also analyzed. (A) Dot plots show the correlation between the proportion of CEACAM+ monocytes from a healthy donor treated with SSc serum and that from patients with SSc obtained at the same time as the serum. Statistical analysis was performed using Spearman’s rank correlation test. (B) The frequency of serum-induced CEACAM+CD14+16- monocytes was compared among HCs (n=7), inactive SSc (n=7), and active SSc (n=7). Each analysis was performed in duplicates. The left figure shows a histogram of CEACAM. (C, D) Data show the frequency of CEACAM1, 3, 5, and 6+ monocytes treated with serum from HCs or patients with dcSSc. Gating of CEACAM6+ monocytes is shown in (C) Data are presented as mean ± standard deviation (SD). P values were calculated using an unpaired t-test. *P < 0.05, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36341379), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CEACAM-6/CD66c by Flow Cytometry

Detection of CEACAM-6/CD66c by Flow Cytometry

SSc serum induced CEACAM6 expression on classical monocytes. CD14+16- monocytes were isolated from a healthy donor and were treated with serum from HCs (n=7) and patients with systemic sclerosis (n=14) for 15 h. The proportion of CEACAM-positive classical monocytes from patients with SSc who provided serum was also analyzed. (A) Dot plots show the correlation between the proportion of CEACAM+ monocytes from a healthy donor treated with SSc serum and that from patients with SSc obtained at the same time as the serum. Statistical analysis was performed using Spearman’s rank correlation test. (B) The frequency of serum-induced CEACAM+CD14+16- monocytes was compared among HCs (n=7), inactive SSc (n=7), and active SSc (n=7). Each analysis was performed in duplicates. The left figure shows a histogram of CEACAM. (C, D) Data show the frequency of CEACAM1, 3, 5, and 6+ monocytes treated with serum from HCs or patients with dcSSc. Gating of CEACAM6+ monocytes is shown in (C) Data are presented as mean ± standard deviation (SD). P values were calculated using an unpaired t-test. *P < 0.05, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36341379), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CEACAM‑6/CD66c Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood granulocytes and HEK293 human embryonic kidney cell line transfected with human CEACAM-6/CD66c and eGFP

Western Blot

1 µg/mL
Sample: Recombinant Human CEACAM‑6/CD66c (Catalog # 3934-CM)

Reviewed Applications

Read 1 review rated 3 using MAB3934 in the following applications:

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-6/CD66c

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific cross‑reacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1-4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and Glycosylphosphatidylinositol (GPI)-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2-4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-Selectin, Galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs -1, -5 (CEA) and -8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments Integrin alpha v beta 3 and beta 2-mediated adhesion, apparently by Src and Caveolin-mediated inside-out Integrin activation (8, 13, 14).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
  3. Barnett, T. et al. (1988) Genomics 3:59.
  4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
  5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
  6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
  7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
  8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
  9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
  10. Kalina, T. et al. (2005) BMC Cancer 5:38.
  11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
  13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
  14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 6

Alternate Names

CD66c, CEACAM6, CEAL, NCA

Entrez Gene IDs

4680 (Human); 107126399 (Cynomolgus Monkey)

Gene Symbol

CEACAM6

UniProt

P40199

Additional CEACAM-6/CD66c Products

Product Documents for Human CEACAM‑6/CD66c Antibody

Certificate of Analysis

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Product Specific Notices for Human CEACAM‑6/CD66c Antibody

For research use only

Citations for Human CEACAM‑6/CD66c Antibody

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  • Human CEACAM-6/CD66c Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HT-29 human colon adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 08/09/2021
    Human CEACAM‑6/CD66c Antibody MAB3934

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