Human Carbohydrate Sulfotransferase 5/CHST5 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF5446AFP680

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG
View all Carbohydrate Sulfotransferase 5/CHST5 Primary Antibodies »

Product Specifications

Specificity

Detects human Carbohydrate Sulfotransferase 5/CHST5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST2, rmCHST5, and rmCHST7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbohydrate Sulfotransferase 5/CHST5

CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha ) is likely a 53‑56 kDa member of the sulfotransfease family of enzymes. It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. It appears to act only on short carbohydrates and transfers sulfate to position # 6 of mucin-associated GlcNAc. Human CHST5 is a 390 amino acid (aa) type II transmembrane protein. It contains a short cytoplasmic segment (aa 1‑9) and a long luminal domain (aa 28‑390) that contains an enzymatic region (aa 180‑358). There is one alternate start site that is 22 aa upstream of the standard site. Humans have a GST4-alpha and -beta gene, the result of gene duplication. Over aa 28‑390, these two gene products share 87% aa identity. Mouse has only one GST4 gene, and it is functionally related to human GST4-beta. Over aa 28‑390, human GST4‑ alpha /CHST5 is 78% aa identical to mouse GST4.

Alternate Names

CHST5, GlcNAc6ST3, GST4-alpha, hIGn6ST, I-GlcNAc6ST

Entrez Gene IDs

23563 (Human); 56773 (Mouse); 307859 (Rat)

Gene Symbol

CHST5

UniProt

Q9GZS9

Additional Carbohydrate Sulfotransferase 5/CHST5 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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FAQs

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail