Human Claudin-1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4618A
Human Claudin-1 APC-conjugated Antibody in Flow Cytometry
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Citations (1)
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Human Claudin-1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human Claudin-1 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 421203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human Claudin-1
Met1-Val211
Accession # O95832
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry. View Larger

Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4618A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-1

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

Entrez Gene IDs
9076 (Human); 12737 (Mouse); 65129 (Rat)
Alternate Names
claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1

Product Datasheets

Citation for Human Claudin-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
    Authors: Giugliano S, Petroff M, Warren B, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko M, Gale M, Golden-Mason L, Winn V, Rosen H
    J Immunol, 2015;195(8):3737-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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