Human Claudin-1 Antibody (421203)

Name: Human Claudin-1 Antibody (421203)
Brand: R&D Systems
SKU: MAB4618
Price: 469 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # MAB4618

(1)

Citations (9)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry, FACS

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 421203

View all Claudin-1 Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with human Claudin-1
Met1-Val211
Accession # O95832

Specificity

Stains human Claudin-1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human Claudin-1 Antibody (421203)

Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113).

Immunofluorescent Staining of iPSC-derived Pancreatic Duct-like Organoids

(A) iPSC-derived pancreatic duct-like organoids were fixed and stained with a Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075; green) and Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red) or with a (B) Mouse Anti-Human KRT19 Monoclonal Antibody (Catalog # MAB35061; green) and Rat Anti-Human Claudin-1 Monoclonal Antibody (Catalog # MAB4618; red), followed by secondary antibody staining with the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL009) and NorthernLights 557-conjugated Goat Anti-Rat IgG Antigen Affinity-purified Polyclonal Antibody (Catalog # NL013). Cell nuclei were stained with DAPI (Catalog # 5748; blue) and the images were overlaid. Images were taken using a Leica THUNDER Imaging System (scale bar: 20 mm).

Applications for Human Claudin-1 Antibody (421203)

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: PC‑3 human prostate cancer cell line

Reviewed Applications

Read 1 review rated 4 using MAB4618 in the following applications:

Western Blot (1 Review)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Claudin-1

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.