Human Claudin-1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4618R-100UG
Human Claudin-1 Alexa Fluor® 647-conjugated Antibody in Data
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Human Claudin-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human Claudin-1 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 421203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human Claudin-1
Met1-Val211
Accession # O95832
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Claudin-1 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB4618R, filled histogram) or isotype control antibody (Catalog # IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-1

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

Entrez Gene IDs
9076 (Human); 12737 (Mouse); 65129 (Rat)
Alternate Names
claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1

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