Human CLEC12B Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB11476AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1062714
View all CLEC12B Primary Antibodies »

Product Specifications

Specificity

Detects human CLEC12B in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human CLEC12B Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CLEC12B

C-type lectin domain family 12 member B (CLEC12B) is a member of the C-type lectin-like family of proteins. CLEC12B is widely expressed at low levels in various human tissues except in the brain (1, 2). A truncated version lacking a portion of the carbohydrate-recognition domain (CRD) has been detected in mammary gland, lung and ovary, and was predicted to be nonfunctional (1). CLEC12B is a cell surface receptor that may play a role in viral recognition and modulate signaling cascades due to the presence of an ITIM motif within its cytoplasmic tail (1-3). Human CLEC12B is synthesized as a 276 amino acid (aa) protein that includes a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 212 aa extracellular domain (ECD). Within the ECD, human CLEC12B shares 74% and 70% aa sequence identity with mouse and rat CLEC12B, respectively. The extracellular domain of CLEC12B shows considerable homology to the activating natural killer cell receptor NKG2D, and it antagonizes NKG2D mediated signaling through the ITIM motif (1). CLEC12B may be involved in limiting the activity of monocyte-derived immune cells after cell differentiation and possibly during inflammatory diseases. They play a role in HIV-1, mycobacterial, and Candida infections, and the coevolution of hosts and pathogens (4). Pathogen recognition by C-type lectins triggers signaling pathways that lead to the expression of specific cytokines which subsequently instruct adaptive T helper immune responses (4).

References

  1. Hoffmann, S. et al. (2007) J. Biol. Chem. 282:22370.
  2. Huysamen, C. et al. (2009) FEMS Microbiol. Lett. 290:121.
  3. Monteiro, J.T. and B. Lepenies (2017) Viruses 9:59.
  4. van den Berg, L.M. et al. (2012) Ann N Y Acad Sci. 1253:149.

Long Name

C-type Lectin Domain Family 12, Member B

Alternate Names

Macrophage Antigen H, UNQ5782

Entrez Gene IDs

387837 (Human); 71183 (Mouse); 502900 (Rat)

Gene Symbol

CLEC12B

UniProt

Q2HXU8

Additional CLEC12B Products

Product Documents for Human CLEC12B Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CLEC12B Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

C-type Lectin Family

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