Human CLEC14A APC-conjugated Antibody

Discontinued Product

FAB7436A has been discontinued.
Detection  of CLEC14A in HUVEC Human Cells by Flow Cytometry.
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Citations (1)
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Human CLEC14A APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC14A in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 743940
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC14A
Glu22-Ala397
Accession # Q86T13
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CLEC14A in HUVEC Human Cells by Flow Cytometry. View Larger

Detection of CLEC14A in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human CLEC14A APC‑conjugated Monoclonal Antibody (Catalog # FAB7436A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC14A

CLEC14A (C-type Lectin Domain Family 14 Member A), also known as EGFR5, is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. It is a type I transmembrane protein, apparently expressed in brain and about which little is known. Mature human CLEC14A is 469 amino acids (aa) in length. It contains a 376 aa extracellular region (aa 22-397) and a 72 aa cytoplasmic domain. The extracellular region shows one C-type lectin like domain (aa 32-175) and an EGF-like region (aa 245-287). Over aa 22-397, human CLEC14A shares 66% and 81% aa identity with mouse and canine CLEC14A, respectively.

Long Name
C-type Lectin Domain Family 14, Member A
Entrez Gene IDs
161198 (Human); 66864 (Mouse); 314148 (Rat)
Alternate Names
C14orf27; CEG1; chromosome 14 open reading frame 27; CLEC14A; ClECT and EGF-like domain containing protein; C-type lectin domain family 14 member A; C-type lectin domain family 14, member A; EGF R5; EGFR5; EGFR-5; Epidermal growth factor receptor 5

Product Datasheets

Citation for Human CLEC14A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
    Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
    J. Leukoc. Biol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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