Human CLEC4E Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB8995AFP594

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2455C
View all CLEC4E Primary Antibodies »

Product Specifications

Specificity

Detects human CLEC4E in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human CLEC4E Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CLEC4E

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Human CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, human CLEC4E shares 65% and 68% aa sequence identity with mouse and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

References

  1. Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
  2. Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
  3. Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
  4. Wells, C.A. et al. (2008) J. Immunol. 180:7404.
  5. Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
  6. Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
  7. Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
  8. Ostrop, J. et al. (2015) J. Immunol. 195:2417.
  9. Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
  10. Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
  11. Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.

Long Name

C-type Lectin Domain Family 4, Member E

Alternate Names

CLECSF9, MINCLE

Entrez Gene IDs

26253 (Human); 56619 (Mouse); 450223 (Rat)

Gene Symbol

CLEC4E

UniProt

Q9ULY5

Additional CLEC4E Products

Product Documents for Human CLEC4E Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human CLEC4E Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

C-type Lectin Family

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