Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9928-100
MAB9928-SP
Detection of CTLA-4 in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP by Flow Cytometry.
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Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human CTLA-4 based on Ipilimumab therapeutic antibody.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Ipilimumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu111
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CTLA-4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CTLA-4 in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP by Flow Cytometry. View Larger

Detection of CTLA-4 in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP by Flow Cytometry. HEK293 Human cell line transfected with (A) Human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CTLA-4 (Research Grade Ipilimumab) Monoclonal Antibody (Catalog # MAB9928) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTLA-4

Ipilimumab is a biosimilar directed against the extracellular domain of CTLA‑4. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB10064. The biotinylated antibody (catalog number FAB9928B) can be used in flow cytometry or various assay format to measure CTLA-4 expression and can be detected by conjugated streptavidin.

Long Name
Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs
1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Product Datasheets

FAQs

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Reconstitution Buffers

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