Human DARC Alexa Fluor® 750-conjugated Antibody

R&D Systems | Catalog # FAB4139S

R&D Systems
Discontinued Product
FAB4139S has been discontinued. View all DARC products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 358307
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Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human DARC
Met1-Ser336
Accession # Q16570

Specificity

Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human DARC Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human blood erythrocytes

Spectra Viewer

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DARC

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Long Name

Duffy Antigen, Receptor For Chemokines

Alternate Names

ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy

Entrez Gene IDs

2532 (Human); 13349 (Mouse)

Gene Symbol

ACKR1

UniProt

Additional DARC Products

Product Documents for Human DARC Alexa Fluor® 750-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DARC Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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