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Human DC-LAMP Lumenal Domain PE-conjugated Antibody

Catalog #: FAB4087P Datasheet / COA / SDS

Discontinued Product

FAB4087P has been discontinued.
View all DC-LAMP products.
Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry.
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Human DC-LAMP Lumenal Domain PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑LAMP Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DC‑LAMP Lumenal Domain
Asp21-Thr381
Accession # EAW78337
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DC-LAMP antibody in Human Mature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Goat Anti-Human DC-LAMP Lumenal Domain PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB4087P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-LAMP

DC-LAMP (Dendritic Cell Lysosome-associated Membrane Protein; also known as CD208 and LAMP3) is a 50‑70 kDa member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It has a 254 aa lumenal N‑terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively.

Long Name
Dendritic Cell-lysosome-associated Membrane Glycoprotein
Entrez Gene IDs
27074 (Human); 239739 (Mouse); 303801 (Rat)
Alternate Names
CD208 antigen; CD208; DCLAMP; DC-LAMP; DCLAMPDC-lysosome-associated membrane glycoprotein; LAMP; LAMP3; LAMP-3; lysosomal-associated membrane protein 3DC LAMP; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403; TSC403CD208

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