Human DC-SIGNR/CD299 Fluorescein-conjugated Antibody

FAB162F has been discontinued. View all DC-SIGNR/CD299 products.
Clone 120604 was used by HLDA to establish CD designation
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human DC‑SIGNR/CD299.
  • Source
    Monoclonal Mouse IgG2B Clone # 120604
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
    Accession # Q9H2X3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example

Detection of DC‑SIGNR/CD299 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGNR/CD299 by Flow Cytometry. NIH‑3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299 was stained with Mouse Anti-Human DC‑SIGNR/CD299 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB162F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DC-SIGNR/CD299

Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin (DC-SIGN or CD299) and DC-SIGN Related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1-4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

  • References:
    1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
    2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
    3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
    4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
    5. Feinberg, H. et al. (2001) Science 294:2163.
  • Long Name:
    Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
  • Entrez Gene IDs:
    10332 (Human)
  • Alternate Names:
    CD209L; CD209L1; CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2; CD299 antigenMGC129964; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DC-SIGN2CD209 antigen-like protein 1; DCSIGNR; DC-SIGNR; DC-SIGNRDC-SIGN-related protein; DCSIGNRMGC47866; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; HP10347; liver/lymph node-specific ICAM-3 grabbing non-integrin; Liver/lymph node-specific ICAM-3-grabbing non-integrin; LSIGN; L-SIGN; LSIGNC-type lectin domain family 4 member M; mannose binding C-type lectin DC-SIGNR
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