Mouse IgG2B Fluorescein‑conjugated Control Antibody

(6 citations)
(1 Review)
  
  • Specificity
    Selected for use as mouse IgG2B isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG2B Clone # 133303
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry U937 human histiocytic lymphoma cell line was stained withMouse Anti-Human SIRP alpha /CD172a Fluorescein-conjugated Monoclonal Antibody (Catalog #FAB4546F,filled histogram) or Mouse IgG Fluorescein -conjugated Isotype Control Antibody
(Catalog # IC0041F, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  2. Expression, Regulation, and Function of Atypical Chemerin Receptor CCRL2 on Endothelial Cells.
    Authors: Monnier J, Lewen S, O'Hara E, Huang K, Tu H, Butcher EC, Zabel BA
    J. Immunol., 2012;189(2):956-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  3. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
    Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
    Innate Immun, 2012;18(3):531-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  4. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011;187(2):748-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  5. Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission.
    Authors: Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM
    Eur. J. Immunol., 2009;39(5):1288-300.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  6. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005;11(18):6686-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
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Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 750-conjugated Isotype Control

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Human Anonymous 07/07/2017
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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

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