Human EN‑RAGE/S100A12 PE‑conjugated Antibody

R&D Systems | Catalog # FAB1052P

R&D Systems
Discontinued Product
FAB1052P has been discontinued. View all EN-RAGE/S100A12 products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
View all EN-RAGE/S100A12 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EN-RAGE/S100A12
Met1-Glu92
Accession # P80511

Specificity

Detects human EN-RAGE/S100A12 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human EN‑RAGE/S100A12 PE‑conjugated Antibody

Detection of EN-RAGE/S100A12 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of EN‑RAGE/S100A12 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Goat Anti-Human EN-RAGE/S100A12 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1052P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EN‑RAGE/S100A12 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EN-RAGE/S100A12

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (Soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1-3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4-6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11-14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11-16).

References

  1. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
  2. Leclerc, E. et al. (2009) Biochim. Biophys. Acta 1793:993.
  3. Wicki, R. et al. (1996) Cell Calcium 20:459.
  4. Moroz, O.V. et al. (2009) BMC Biochem. 10:11.
  5. Miranda, L.P. et al. (2001) FEBS Lett. 488:85.
  6. Vogl, T. et al. (1999) J. Biol. Chem. 274:25291.
  7. Xie, J. et al. (2007) J. Biol. Chem. 282:4218.
  8. Goyette, J. et al. (2009) J. Immunol. 183:593.
  9. Hatakeyama, T. et al. (2004) Eur. J. Biochem. 271:3765.
  10. Hofmann, M.A. et al. (1999) Cell 97:889.
  11. Yang, Z. et al. (2001) J. Leukoc. Biol. 69:986.
  12. Rouleau, P. et al. (2003) Clin. Immunol. 107:46.
  13. Yan, W.X. et al. (2008) J. Biol. Chem. 283:13035.
  14. Yang, Z. et al. (2007) J. Allergy Clin. Immunol. 119:106.
  15. Mikkelsen, S.E. et al. (2001) J. Neurochem. 79:767.
  16. Fuellen, G. et al. (2004) OMICS 8:334.

Long Name

Extracellular Newly Identified RAGE-binding Protein

Alternate Names

CAAF1, CAAFI, CAGC, CAGCS100, Calgranulin C, ENRAGE, MRP6, S100A12

Entrez Gene IDs

6283 (Human)

Gene Symbol

S100A12

UniProt

P80511

Additional EN-RAGE/S100A12 Products

Product Documents for Human EN‑RAGE/S100A12 PE‑conjugated Antibody

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Product Specific Notices for Human EN‑RAGE/S100A12 PE‑conjugated Antibody

For research use only

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