Human EphB3 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human EphB3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) EphA3, rhEphA4, EphB2, or recombinant mouse EphB3 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 647354
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EphB3
    Accession # P54753
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EphB3 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human EphB3 PE‑conjugated Monoclonal Antibody (Catalog # FAB56671P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EphB3

EphB3 (Erythropoietin-producing Hepatocellular Carcinoma-3), also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 110-130 kDa type I transmembrane glycoprotein member of the Ephrin receptor subfamily, tyrosine protein kinase family of molecules. The Eph proteins have an A and B class that are distinguished by Ephrin ligand binding preference and a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and inflammation. The 526 amino acid (aa) extracellular domain (ECD) of mature human EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif. Within the ECD, human EphB3 shares 96% aa sequence identity with mouse and rat EphB3. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin. EphB3 also interacts in cis with the receptor tyrosine kinase Ryk. Activation of its kinase domain is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology. EphB3 is widely expressed during development and in the adult where it shows a complementary tissue distribution to the Ephrin-B ligands. Cells known to express EphB3 constitutively or inducibly include Langerhans cells, osteoblasts, oligodendroglia, astrocytes, neural stem cell progenitors, cortical thymic epithelium, duodenal Brunner's gland epithelium and gastric parietal cells. EphB3 function is important in vascular, nervous system, thymocyte, and palate development. It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons. Notably, during pancreas development, EphB3 is reportedly a definitive biomarker for fetal islet precursor cells.

  • Long Name:
    Eph Receptor B3
  • Entrez Gene IDs:
    2049 (Human); 13845 (Mouse)
  • Alternate Names:
    Cek10; EC 2.7.10; EC; EK2; EPH receptor B3; EphB3; EPH-like kinase 2; EPH-like tyrosine kinase-2; ephrin type-B receptor 3; ETK2Hek2; Hek2; human embryo kinase 2; Mdk5; Sek4; Tyro6; Tyrosine-protein kinase TYRO6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 10  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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