Human Epiregulin PE-conjugated Antibody

IC14251P has been discontinued and is replaced by IC14251TIC14251G.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Epiregulin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Betacellulin, rhEGF, recombinant mouse Epiregulin, or rhHB-EGF is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 183629
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Epiregulin
    Val63-Leu108
    Accession # O14944
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of Epiregulin in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human Epiregulin PE‑conjugated Monoclonal Antibody (Catalog # IC14251P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Epiregulin

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. All EGF family members are synthesized as transmembrane precursors and are converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The human epiregulin cDNA encodes a 169 amino acid (aa) residues transmembrane precursor with a 29 aa signal peptide, a 21 aa transmembrane domain and a 21 aa cytoplasmic domain. The putative soluble mature Epiregulin comprising the EGF-like domain (aa residues 64‑104) is formed by proteolytic removal of the propeptide regions (2). There is 85% aa sequence homology between human and mouse epiregulins. Epiregulin is expressed primarily in the placenta and macrophages (3). High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGFR (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members (4). Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells (5, 6). Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy (7).

  • References:
    1. Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
    2. Toyoda, H. et al. (1997) Biochem. J. 326:69.
    3. Komurasaki, T. et al. (1997) Oncogene 15:2841.
    4. Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
    5. Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
    6. Mishre, R. et al. (2002) Am. J. Physiol. Renal. Physiol. 283:F1151.
    7. Das, S.K. et al. (1997) Dev. Biol. 190:178.
  • Entrez Gene IDs:
    2069 (Human); 13874 (Mouse)
  • Alternate Names:
    Epiregulin; ER; EREG; proepiregulin
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