Human ERMAP APC‑conjugated Antibody

R&D Systems | Catalog # FAB49281A

R&D Systems
Discontinued Product
FAB49281A has been discontinued. View all ERMAP products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 767505
View all ERMAP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ERMAP
His30-Ala155
Accession # Q96PL5

Specificity

Detects human ERMAP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human ERMAP APC‑conjugated Antibody

Detection of ERMAP antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of ERMAP in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ERMAP APC-conjugated Monoclonal Antibody (Catalog # FAB49281A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ERMAP APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ERMAP

ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60-66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors, and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30-155) that contains one V-type Ig-like domain (aa 30-140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103-475. Over aa 30-155, human ERMAP shares 56% aa sequence identity with mouse ERMAP. In contrast to the human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.

Long Name

Erythroblast Membrane-associated Protein

Alternate Names

RD, SC

Entrez Gene IDs

114625 (Human); 27028 (Mouse); 298485 (Rat)

Gene Symbol

ERMAP

UniProt

Q96PL5

Additional ERMAP Products

Product Documents for Human ERMAP APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human ERMAP APC‑conjugated Antibody

For research use only

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