Human Erythropoietin R Alexa Fluor® 700-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Epo R in ELISAs. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) Epo, rhTpo R, or rmEpo R.
  • Source
    Monoclonal Mouse IgG1 Clone # 38421
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
    Ala25-Pro250
    Accession # P19235
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF‑1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3072N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Erythropoietin R
Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.
  • Long Name:
    Erythropoietin Receptor
  • Entrez Gene IDs:
    2057 (Human); 13857 (Mouse)
  • Alternate Names:
    EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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