FAP (also known as seprase) is a transmembrane serine protease, and a soluble and enzymatically active form of FAP known as antiplasmin-cleaving enzyme (APCE) circulates in human plasma. FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV/CD26, which is specific for N-terminal Xaa-Pro sequences. FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion. In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human FAP QuicKit ELISA
Product Specifications
Measurement
Detection Method
Conjugate
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 283 | 1511 | 315 | 1835 |
| Standard Deviation | 14.1 | 103 | 34.4 | 84.2 |
| CV% | 5.0 | 6.8 | 10.9 | 4.6 |
Recovery for Human FAP QuicKit ELISA
The recovery of human FAP spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 94-108 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FAP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human FAP QuicKit ELISA
Human FAP Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Fibroblast Activation Protein alpha/FAP
Alternate Names
Gene Symbol
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Product Documents for Human FAP QuicKit ELISA
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FAP QuicKit ELISA
For research use only
Related Research Areas
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