Human Fc gamma  RI/CD64 Fluorescein-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human Fc gamma RI/CD64 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) Fc gamma  RI, rmFc gamma  RII, recombinant human (rh) Fc gamma  RIIA, rhFc gamma  RIIB, or rhFc gamma  RIIIB.
  • Source
    Monoclonal Mouse IgG1 Clone # 276426
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Fc gamma  RI/CD64
    Accession # P12314.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Fc gamma  RI/CD64 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma  RI/CD64 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB12571F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in multiple isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8 - 10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins are lower affinity receptors (~10-6 - 10-7 M) that only recognize IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains, indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis. Over amino acids (aa) 16-288, human and mouse share 72% aa sequence identity.

  • Long Name:
    Fc gamma Receptor I
  • Entrez Gene IDs:
    2209 (Human); 14129 (Mouse); 102147198 (Cynomolgus Monkey)
  • Alternate Names:
    CD64 antigen; CD64; Fc fragment of IgG, high affinity Ia, receptor (CD64); FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FCGR1A; FcgRI; FCRI; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Whose Gene Is It Anyway? The Effect of Preparation Purity on Neutrophil Transcriptome Studies.
    Authors: Thomas H, Moots R, Edwards S, Wright H
    PLoS ONE, 2015;10(9):e0138982.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 9  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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