Mouse IgG1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
IC002F
Detection of Mouse IgG Control by Flow Cytometry
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Product Details
Citations (19)
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Mouse IgG1 Fluorescein-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human IFN-? R1/CD119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB673F, filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1

Product Datasheets

Citations for Mouse IgG1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
    Authors: MY Hsiao, PC Lin, WH Liao, WS Chen, CH Hsu, CK He, YW Wu, A Gefen, M Iafisco, L Liu, FH Lin
    Int J Mol Sci, 2019;20(14):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Activator of G-protein signaling 8 is involved in VEGF-induced choroidal neovascularization
    Authors: H Hayashi, AA Mamun, M Takeyama, A Yamamura, M Zako, R Yagasaki, T Nakahara, M Kamei, M Sato
    Sci Rep, 2019;9(1):1560.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder
    Authors: L Shi, YJ Li, GC Dai, YC Lin, G Li, C Wang, H Chen, YF Rui
    Stem Cell Res Ther, 2019;10(1):27.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Allogeneic multipotent mesenchymal stromal cell sheet transplantation promotes healthy healing of wounds caused by zoledronate and dexamethasone in canine mandibular bones
    Authors: N Kaibuchi, T Iwata, S Onizuka, K Yano, Y Tsumanuma, M Yamato, T Okano, T Ando
    Regen Ther, 2019;10(0):77-83.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2
    Authors: CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. TGF-?1-induced chondrogenesis of bone marrow mesenchymal stem cells is promoted by low-intensity pulsed ultrasound through the integrin-mTOR signaling pathway
    Authors: P Xia, X Wang, Y Qu, Q Lin, K Cheng, M Gao, S Ren, T Zhang, X Li
    Stem Cell Res Ther, 2017;8(1):281.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  7. Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro
    PLoS ONE, 2016;11(11):e0165926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  9. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  10. Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs.
    Authors: Hashimoto, Noriaki, Tsunedomi, Ryouichi, Yoshimura, Kiyoshi, Watanabe, Yusaku, Hazama, Shoichi, Oka, Masaaki
    BMC Cancer, 2014;14(0):722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  11. The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells.
    Authors: Wilhelmsen K, Khakpour S, Tran A, Sheehan K, Schumacher M, Xu F, Hellman J
    J Biol Chem, 2014;289(19):13079-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing.
    Authors: Wei F, Leung K, Li G, Qin J, Chow S, Huang S, Sun M, Qin L, Cheung W
    PLoS ONE, 2014;9(9):e106722.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation.
    Authors: Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G
    J Immunol, 2014;192(2):741-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  14. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011;187(2):748-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  15. Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission.
    Authors: Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM
    Eur. J. Immunol., 2009;39(5):1288-300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  16. Human mesenchymal stem cells inhibit neutrophil apoptosis: a model for neutrophil preservation in the bone marrow niche.
    Authors: Raffaghello L, Bianchi G, Bertolotto M, Montecucco F, Busca A, Dallegri F, Ottonello L, Pistoia V
    Stem Cells, 2007;26(1):151-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  17. Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
    Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
    J. Mol. Biol., 2006;367(1):25-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005;11(18):6686-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  19. Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
    Authors: Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
    Crit. Care Med., 2005;33(6):1359-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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