Home / IgG1 / Mouse IgG1 Fluorescein-conjugated Antibody

Mouse IgG1 Fluorescein-conjugated Antibody

Catalog #: IC002F
24 Citations 2 Reviews Datasheet / COA / SDS
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IC002F
Detection of Mouse IgG Isotype Control by Flow Cytometry
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Product Details
Citations (24)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG1 Fluorescein-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Mouse Anti-Human IFN-gamma R1/CD119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB673F, filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Mouse IgG1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. IL-17-induced dimerization of IL-17RA drives the formation of the IL-17 signalosome to potentiate signaling
    Authors: A Goepfert, C Barske, S Lehmann, E Wirth, J Willemsen, JE Gudjonsson, NL Ward, MK Sarkar, R Hemmig, F Kolbinger, JM Rondeau
    Cell Reports, 2022;41(3):111489.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Chemokine (C-C Motif) Ligand 8 and Tubulo-Interstitial Injury in Chronic Kidney Disease
    Authors: J Lee, Y Lee, KH Kim, DK Kim, KW Joo, SJ Shin, YS Kim, SH Yang
    Cells, 2022;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  3. Anti-inflammatory Effects of Alcohol Are Associated with JNK-STAT3 Downregulation in an In Vitro Inflammation Model in HepG2 Cells
    Authors: K Mörs, R Sturm, JA Hörauf, S Kany, P Cavalli, J Omari, M Powerski, A Surov, I Marzi, AJ Nowak, B Relja
    Disease Markers, 2021;2021(0):6622701.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  4. LasR-deficient Pseudomonas aeruginosa variants increase airway epithelial mICAM-1 expression and enhance neutrophilic lung inflammation
    Authors: LC Hennemann, SL LaFayette, JK Malet, P Bortolotti, T Yang, GA McKay, D Houle, D Radzioch, S Rousseau, D Nguyen
    PloS Pathogens, 2021;17(3):e1009375.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Targeting the ABC transporter ABCB5 sensitizes glioblastoma to temozolomide-induced apoptosis through a cell-cycle checkpoint regulation mechanism
    Authors: CAA Lee, P Banerjee, BJ Wilson, S Wu, Q Guo, G Berg, S Karpova, A Mishra, JW Lian, J Tran, M Emmerich, GF Murphy, MH Frank, NY Frank
    J. Biol. Chem., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Allogeneic multipotent mesenchymal stromal cell sheet transplantation promotes healthy healing of wounds caused by zoledronate and dexamethasone in canine mandibular bones
    Authors: N Kaibuchi, T Iwata, S Onizuka, K Yano, Y Tsumanuma, M Yamato, T Okano, T Ando
    Regen Ther, 2019;10(0):77-83.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor
    Authors: MY Hsiao, PC Lin, WH Liao, WS Chen, CH Hsu, CK He, YW Wu, A Gefen, M Iafisco, L Liu, FH Lin
    Int J Mol Sci, 2019;20(14):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Activator of G-protein signaling 8 is involved in VEGF-induced choroidal neovascularization
    Authors: H Hayashi, AA Mamun, M Takeyama, A Yamamura, M Zako, R Yagasaki, T Nakahara, M Kamei, M Sato
    Sci Rep, 2019;9(1):1560.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder
    Authors: L Shi, YJ Li, GC Dai, YC Lin, G Li, C Wang, H Chen, YF Rui
    Stem Cell Res Ther, 2019;10(1):27.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Bone morphogenetic protein (BMP) 9 and BMP10 enhance tumor necrosis factor-?-induced monocyte recruitment to the vascular endothelium mainly via activin receptor-like kinase 2
    Authors: CG Mitrofan, SL Appleby, GB Nash, Z Mallat, ER Chilvers, PD Upton, NW Morrell
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. TGF-?1-induced chondrogenesis of bone marrow mesenchymal stem cells is promoted by low-intensity pulsed ultrasound through the integrin-mTOR signaling pathway
    Authors: P Xia, X Wang, Y Qu, Q Lin, K Cheng, M Gao, S Ren, T Zhang, X Li
    Stem Cell Res Ther, 2017;8(1):281.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  12. Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro
    PLoS ONE, 2016;11(11):e0165926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  13. Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  14. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  15. ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation.
    Authors: Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G
    J Immunol, 2014;192(2):741-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  16. The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells.
    Authors: Wilhelmsen K, Khakpour S, Tran A, Sheehan K, Schumacher M, Xu F, Hellman J
    J Biol Chem, 2014;289(19):13079-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Cancer stem-like sphere cells induced from de-differentiated hepatocellular carcinoma-derived cell lines possess the resistance to anti-cancer drugs.
    Authors: Hashimoto, Noriaki, Tsunedomi, Ryouichi, Yoshimura, Kiyoshi, Watanabe, Yusaku, Hazama, Shoichi, Oka, Masaaki
    BMC Cancer, 2014;14(0):722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  18. Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing.
    Authors: Wei F, Leung K, Li G, Qin J, Chow S, Huang S, Sun M, Qin L, Cheung W
    PLoS ONE, 2014;9(9):e106722.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011;187(2):748-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  20. Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission.
    Authors: Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM
    Eur. J. Immunol., 2009;39(5):1288-300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  21. Human mesenchymal stem cells inhibit neutrophil apoptosis: a model for neutrophil preservation in the bone marrow niche.
    Authors: Raffaghello L, Bianchi G, Bertolotto M, Montecucco F, Busca A, Dallegri F, Ottonello L, Pistoia V
    Stem Cells, 2007;26(1):151-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  22. Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
    Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
    J. Mol. Biol., 2006;367(1):25-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005;11(18):6686-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  24. Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
    Authors: Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
    Crit. Care Med., 2005;33(6):1359-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse IgG1 Fluorescein-conjugated Antibody

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Mouse IgG1 Fluorescein-conjugated Antibody
By Anonymous on 02/02/2023
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

Use as Iso-type Ctrl

Flow Cytometry Mouse IgG1 Fluorescein-conjugated Antibody IC002F
Mouse IgG1 Fluorescein-conjugated Antibody
By Anonymous on 01/08/2020
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human