Human FGFR1 alpha (IIIc) Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB11336AFP594

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1058809
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Product Specifications

Specificity

Detects human FGFR1 alpha (IIIc) in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human FGFR1 alpha (IIIc) Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FGFR1 alpha

Fibroblast growth factor receptor 1 (FGFR1) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing an extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and cytoplasmic split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR1 shares 98% amino acid sequence identity with mouse FGFR1. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR1, alternative splicing of the ECD generates FGFR1A, FGFR1B, and FGFR1G isoforms of the receptor with the A isoform containing three Ig domains, while the B and G isoforms lack the N‑terminal IgI domain (3). Additional splicing of the IgIII domain, results in IIIa, IIIb, or IIIc isoforms (3). Only the alpha isoform has been identified for FGFR3 and FGFR4 and FGFR4 also lacks the IIIb and IIIc splicing events (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (5). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (6). FGFR1 the most abundant FGFR and is widely expressed in many adult human tissues, but the splice variants display distinct tissue-specific differences with IIIc splice variants expressed in mesenchymal tissue (4, 7, 8). Mutations in FGFR1 or misregulation of FGFR1 mediated signaling is found in multiple diseases, with FGFR1A(IIIc) specifically upregulated, from breast and pancreatic cancer to Pfeiffer syndrome and osteoarthritis (4, 9-11). A soluble version of the FGFR1A(IIIc) splice variant has shown anti-angiogenic and anti-proliferative properties in multiple cancer cell line models (11).

References

  1. Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip. Rev. Dev. Biol. 4:215.
  2. Zhang, X. et al. (2006) J Biol. Chem. 281:15694.
  3. Ferguson, H.R. et al. (2021) Signaling Cells 10:1201.
  4. Holzmann, K. et al. (2012) J Nucleic. Acids 2012:950508.
  5. Xie, Y. et al. (2020) Sig. Transduct. Target Ther. 5:181.
  6. Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev. Biol. 18:79.
  7. Hughes, S.E. (1997) J. Histochem. Cytochem. 45:1005.
  8. Delezoide, A.L. et al. (1998) Mech. Dev. 77:19.
  9. Yamashita-Sugahara, Y. et al. (2016) Sci. Rep. 6:35908.
  10. Teven, C.M. et al. (2014) Genes Dis. 1:199.
  11. Babina, I. and Turner, N. (2017) Nat. Rev Cancer 17:318.

Long Name

Fibroblast Growth Factor Receptor 1 alpha

Alternate Names

FGF R1a

UniProt

P11362.3

Additional FGFR1 alpha Products

Product Documents for Human FGFR1 alpha (IIIc) Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human FGFR1 alpha (IIIc) Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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