Human FoxF2 Antibody

R&D Systems | Catalog # AF6988

R&D Systems
Discontinued Product
AF6988 has been discontinued. View all FoxF2 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FoxF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxF2
Ala324-Met444
Accession # Q12947

Specificity

Detects human FoxF2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) FoxF1, rhFoxP3, and rhFoxC2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FoxF2 Antibody

Detection of Human FoxF2 antibody by Western Blot.

Detection of Human FoxF2 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxF2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human FoxF2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxF2

FoxF2 (Forkhead box protein F2; also FREAC-2 and FKHL6) is a 42-46 kDa member of the winged helix transcription factor gene family. It is a transcriptional activator that is expressed in embryonic mesenchyme, inducing the synthesis if BMP4 and ECM. In the adult, it is expressed in placenta and lung, specifically in type II pneumocytes plus bronchiolar epithelium, inducing the synthesis of C10 and surfactant proteins B and C. Human FoxF2 is 444 amino acids (aa) in length. It contains one poly-Ala repeat (aa 33-41), a poly-Ser region (aa 46-67), and a poly-Gly segment (aa 77-84), followed by a forkhead DNA binding domain (aa 99-190) and three homopolymeric "runs" involving His (aa 262-272), Gly (aa 301-306) and Ser (aa 312-315). Over aa 324-444, human FoxF2 shares 99% aa identity with mouse FoxF2.

Long Name

Forkhead Box F2

Alternate Names

FKHL6, FREAC2

Entrez Gene IDs

2295 (Human); 14238 (Mouse)

Gene Symbol

FOXF2

UniProt

Q12947 (Human)

Additional FoxF2 Products

Product Documents for Human FoxF2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FoxF2 Antibody

For research use only

Citations for Human FoxF2 Antibody

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