Human FoxF2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FoxF2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) FoxF1, rhFoxP3, and rhFoxC2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FoxF2
    Ala324-Met444
    Accession # Q12947
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FoxF2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxF2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxF2

FoxF2 (Forkhead box protein F2; also FREAC-2 and FKHL6) is a 42-46 kDa member of the winged helix transcription factor gene family. It is a transcriptional activator that is expressed in embryonic mesenchyme, inducing the synthesis if BMP4 and ECM. In the adult, it is expressed in placenta and lung, specifically in type II pneumocytes plus bronchiolar epithelium, inducing the synthesis of C10 and surfactant proteins B and C. Human FoxF2 is 444 amino acids (aa) in length. It contains one poly-Ala repeat (aa 33-41), a poly-Ser region (aa 46-67), and a poly-Gly segment (aa 77-84), followed by a forkhead DNA binding domain (aa 99-190) and three homopolymeric "runs" involving His (aa 262-272), Gly (aa 301-306) and Ser (aa 312-315). Over aa 324-444, human FoxF2 shares 99% aa identity with mouse FoxF2.

  • Long Name:
    Forkhead Box F2
  • Entrez Gene IDs:
    2295 (Human); 14238 (Mouse)
  • Alternate Names:
    FKHL6; FKHL6forkhead box protein F2; forkhead box F2; forkhead-like 6; Forkhead-related activator 2; Forkhead-related protein FKHL6; Forkhead-related transcription factor 2; FoxF2; FREAC2; FREAC2FREAC-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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