Human FoxF2 Antibody

Catalog #: AF6988
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6988
AF6988-SP

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Detection of Human FoxF2 by Western Blot.

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Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxF2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) FoxF1, rhFoxP3, and rhFoxC2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxF2
Ala324-Met444
Accession # Q12947

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FoxF2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human FoxF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6988) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxF2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxF2

FoxF2 (Forkhead box protein F2; also FREAC-2 and FKHL6) is a 42-46 kDa member of the winged helix transcription factor gene family. It is a transcriptional activator that is expressed in embryonic mesenchyme, inducing the synthesis if BMP4 and ECM. In the adult, it is expressed in placenta and lung, specifically in type II pneumocytes plus bronchiolar epithelium, inducing the synthesis of C10 and surfactant proteins B and C. Human FoxF2 is 444 amino acids (aa) in length. It contains one poly-Ala repeat (aa 33-41), a poly-Ser region (aa 46-67), and a poly-Gly segment (aa 77-84), followed by a forkhead DNA binding domain (aa 99-190) and three homopolymeric "runs" involving His (aa 262-272), Gly (aa 301-306) and Ser (aa 312-315). Over aa 324-444, human FoxF2 shares 99% aa identity with mouse FoxF2.

Long Name

Forkhead Box F2

Entrez Gene IDs

2295 (Human); 14238 (Mouse)

Alternate Names

FKHL6; FKHL6forkhead box protein F2; forkhead box F2; forkhead-like 6; Forkhead-related activator 2; Forkhead-related protein FKHL6; Forkhead-related transcription factor 2; FoxF2; FREAC2; FREAC2FREAC-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Forkhead Domain Transcription Factors

Citations for Human FoxF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Stromal FOXF2 suppresses prostate cancer progression and metastasis by enhancing antitumor immunity
Authors: D Jia, Z Zhou, OJ Kwon, L Zhang, X Wei, Y Zhang, M Yi, MP Roudier, MC Regier, R Dumpit, PS Nelson, M Headley, L True, DW Lin, C Morrissey, CJ Creighton, L Xin
Nature Communications, 2022;13(1):6828.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
Authors: J Xu, H Liu, Y Lan, M Adam, DE Clouthier, S Potter, R Jiang
Elife, 2019;8(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P