Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human FYB/ADAP/SLAP130 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 460107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FYB/ADAP/SLAP130
    Ala2-Gly274
    Accession # O15117
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of FYB/ADAP/SLAP130 in Jurkat Human Cell Line by Flow Cytometry Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # IC38571V, filled histogram) or isotype control antibody (Catalog # IC0041V, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FYB/ADAP/SLAP130
Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively-spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.
  • Long Name:
    FYN Binding Protein (FYB-120/130)
  • Entrez Gene IDs:
    2533 (Human); 23880 (Mouse); 499537 (Rat)
  • Alternate Names:
    ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein

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