Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
IC38571V-100UG
Detection of FYB/ADAP/SLAP130 in Jurkat Human Cell Line by Flow Cytometry
1 Image
Product Details
FAQs
Reviews

Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FYB/ADAP/SLAP130 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 460107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FYB/ADAP/SLAP130
Ala2-Gly274
Accession # O15117
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of FYB/ADAP/SLAP130 in Jurkat Human Cell Line by Flow Cytometry View Larger

Detection of FYB/ADAP/SLAP130 in Jurkat Human Cell Line by Flow Cytometry Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # IC38571V, filled histogram) or isotype control antibody (Catalog # IC0041V, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FYB/ADAP/SLAP130

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively-spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.

Long Name
FYN Binding Protein (FYB-120/130)
Entrez Gene IDs
2533 (Human); 23880 (Mouse); 499537 (Rat)
Alternate Names
ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Antibody and earn rewards!

Have you used Human FYB/ADAP/SLAP130 Alexa Fluor® 405-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review